β-catenin is critical for dendritic morphogenesis
β-catenin is critical for dendritic morphogenesis
复制标题
DOI：
10.1038/nn1132
复制
发表时间：
2003-11-01
影响因子：
25
通讯作者：
Malenka, RC
中科院分区：
文献类型：
--
作者：
Yu, X;Malenka, RC
树突状突起的调控生长和分枝对功能性神经元网络的形成至关重要。在此，我们发现β-连环蛋白是树突状细胞形态发生的关键介体。我们发现，增加细胞内β-连环蛋白和钙粘素/连环蛋白复合体的其他成员，即N-钙粘连蛋白和N-连环蛋白，可以增强大鼠海马神经元中树突状树枝的形成，这一效应不需要Wnt/β-连环蛋白依赖的转录。相反，隔离β-连环蛋白的蛋白质减少了树突状分支尖端的数量和总的树突分支长度。去极化诱导的树突状细胞生长需要β-连环蛋白和增加Wnt的释放。这些结果证实Wnt/β-catenin信号是树突状细胞发育的重要介质，并提示细胞内钙粘附素/连环素复合体的水平是树突状细胞形态发生关键阶段的限制因素。
Regulated growth and arborization of dendritic processes are critical to the formation of functional neuronal networks. Here we identify beta-catenin as a critical mediator of dendritic morphogenesis. We found that increasing the intracellular levels of beta-catenin and other members of the cadherin/catenin complex, namely N-cadherin and N-catenin, enhances dendritic arborization in rat hippocampal neurons, an effect that does not require Wnt/beta-catenin-dependent transcription. Conversely, proteins that sequester beta-catenin decreased dendritic branch tip number and total dendritic branch length. Enhancement of dendritic growth elicited by depolarization requires beta-catenin and increased Wnt release. These results identify Wnt/beta-catenin signaling as an important mediator of dendritic development and suggest that the intracellular level of the cadherin/catenin complex is a limiting factor during critical stages of dendritic morphogenesis.