Realistic three-dimensional epithelial tissue phantoms for biomedical optics
Realistic three-dimensional epithelial tissue phantoms for biomedical optics
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DOI：
10.1117/1.1427052
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发表时间：
2002-01-01
影响因子：
3.5
通讯作者：
Richards-Kortum, R
中科院分区：
文献类型：
--
作者：
Sokolov, K;Galvan, J;Richards-Kortum, R
我们介绍了新的现实三维组织幻影，它可以帮助了解人类上皮细胞的光学特性，以及与不典型增生癌序列的光学签名。该模型基于使用人类上皮的主要特征成分逐步多层重建上皮组织。每个连续步骤旨在增加真实的组织和体模之间的相似性。我们首先对基质层进行建模，基质层主要由胶原纤维束网络组成。建立了由单独的胶原基质和存在包埋的宫颈细胞的胶原基质组成的子宫内膜。对它们的形态和荧光特性进行了研究，并与宫颈上皮进行了比较。我们表明，幻影类似的显微结构和光学特性的人类上皮组织，我们还表明，建议幻影提供了一个机会，研究不同的组织成分的光学特性的变化，由于它们之间的相互作用或外源性因素。(C)2002年，美国光电仪器工程师学会。
We introduce new realistic three-dimensional tissue phantoms which can help to understand the optical properties of human epithelium as well as the optical signatures associated with the dysplasia to carcinoma sequence. The phantoms are based on a step by step multilayer reconstitution of the epithelial tissue using main components characteristic for the human epithelium. Each consecutive step is aimed to increase the similarity between real tissue and a phantom. We began by modeling the stromal layer which predominantly consists of a network of collagen bundles. Phantoms consisting of a collagen matrix alone and in the presence of embedded cervical cells were created. Their morphology and fluorescence properties were studied and were compared with those of cervical epithelium. We show that the phantoms resemble the microstructure and the optical properties of the human epithelial tissue, We also demonstrate that the proposed phantoms provide an opportunity to study changes in optical properties of different tissue components as a result of their interactions with each other or exogenous factors. (C) 2002 Society of Photo-Optical Instrumentation Engineers.