NeuroD is required for differentiation of the granule cells in the cerebellum and hippocampus
NeuroD is required for differentiation of the granule cells in the cerebellum and hippocampus
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DOI：
10.1101/gad.13.13.1647
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发表时间：
1999-07-01
影响因子：
10.5
通讯作者：
Lee, JE
中科院分区：
文献类型：
--
作者：
Miyata, T;Maeda, T;Lee, JE
NeuroD是一种bHLH转录因子，参与神经元和胰腺β细胞的分化。NeuroD基因缺失的小鼠在出生后不久就死于严重的新生儿糖尿病。为了检查这些小鼠是否存在出生后神经元表型，我们通过在胰岛素启动子下引入编码小鼠neuroD基因的转基因将其从新生儿致死性中拯救出来。这些小鼠存活至成年，但由于小脑和海马的颗粒层中的神经元缺陷而显示出严重的神经学表型。我们在这里表明，NeuroD是这些出生后产生的微神经元进行适当的分化所必需的，缺乏这种分化会导致细胞死亡。
NeuroD, a bHLH transcription factor, is implicated in differentiation of neurons and pancreatic beta cells. NeuroD-null mice die shortly after birth due to severe neonatal diabetes. To examine if there is postnatal neuronal phenotype in these mice, we rescued them from neonatal lethality by introducing a transgene encoding the mouse neuroD gene under the insulin promoter. These mice survive to adulthood but display severe neurological phenotype due to neuronal deficit in the granule layers of the cerebellum and hippocampus. We show here that NeuroD is required for these postnatally generated microneurons to undergo proper differentiation, the absence of which results in cell death.