The role of the coat protein region in symptom formation on Physalis floridana varies between PVY strains
The role of the coat protein region in symptom formation on Physalis floridana varies between PVY strains
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DOI：
10.1016/j.virusres.2007.03.023
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发表时间：
2007-07-01
期刊：
影响因子：
5
通讯作者：
Balazs, Ervin
中科院分区：
文献类型：
--
作者：
Bukovinszki, Agnes;Goetz, Reinhard;Balazs, Ervin
由Jakab等人[Jakab，G.，Droz，E.，Brigneti，G.，Baulcombe，D.，Malnoe，，P.，1997.来自马铃薯Y病毒瑞士坏死分离物PVY-N605全长cDNA克隆的感染性体内和体外转录物。J.将军·维罗尔78，3141-3145]通过将三个内含子插入到脓毒症毒性基因中来稳定。利用该克隆，通过体外重组构建杂交病毒。PVY-N/NTN和PVY-N/O嵌合体在PVYN遗传背景下分别携带NIb的3'端、PVYNTN和PVYO的整个CP和3' UTR区域。通过对交换的区域进行重新测序，证明克隆在几次传代后是稳定的。这两种杂交病毒在粒子轰击实验中表现出降低的感染性，但它们适合于进一步的机械植物接种。在六种宿主植物中的五种中，接种两种嵌合体和三种亲本菌株，嵌合体产生与PVYN相似的症状。而酸浆则表现出不同的症状模式。在该物种中，由N/O杂种引起的症状与3 'NIb-CP供体PVYO菌株的症状相似，而不是背景（PVYN）的症状。结果表明，症状决定因素可能是不同的，甚至在同一病毒种株在特定的主机。(C)2007 Elsevier B. V.保留所有权利。
The Potato virus Y (PVY) cDNA full-length clone created by Jakab et al. [Jakab, G., Droz, E., Brigneti, G., Baulcombe, D., Malnoe,, P., 1997. Infectious in vivo and in vitro transcripts from a full-length cDNA clone of PVY-N605, a Swiss necrotic isolate of potato virus Y. J. Gen. Virol. 78, 3141-3145] was stabilized by inserting three introns into putatively toxic genes. Using this clone, hybrid viruses were constructed by in vitro recombination. The PVY-N/NTN and PVY-N/O chimeras carried the 3' end of NIb, the whole CP and 3'UTR region of PVYNTN and PVYO, respectively, in a PVYN genetic background. The clones proved to be stable after several passages by re-sequencing the exchanged region. Both hybrid viruses showed reduced infectivity in particle bombardment experiments, but they were suitable for further mechanical plant inoculation. In five of the six host plant species, inoculated with the two chimeras and three parental strains, the chimeras produced similar symptoms to those of PVYN. By contrast, Physalis floridana reacted with different pattern of symptoms. In this species, the symptoms caused by the N/O hybrid were similar to those of the 3'NIb-CP-donating PVYO strain, and not to those of the background (PVYN). The results suggest that symptom determinants may be different even between strains of the same virus species in a particular host. (C) 2007 Elsevier B.V. All rights reserved.