Rapid and Easy Multiresidue Method for Analysis of Antibiotics in Meats by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry
Rapid and Easy Multiresidue Method for Analysis of Antibiotics in Meats by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry
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采用超高效液相色谱-串联质谱法快速简便地分析肉类中抗生素的多残留方法
DOI：
10.1021/acs.jafc.5b00170
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发表时间：
2015
影响因子：
6.1
通讯作者：
Y.Yamamoto
中科院分区：
文献类型：
--
作者：
T. Yamaguchi;M. Okihashi;K. Harada;K. Uchida;Y. Konishi;K. Kajimura;K. Hirata;Y.Yamamoto
本研究建立了超高效液相色谱-串联质谱法快速分析肉类中43种抗生素的多残留方法。该方法采用分散-固相萃取，可在2 h内分析20个样品，在C18分离柱上进行梯度洗脱，同时测定所有化合物。通过在不同肉制品中进行线性、定量限(LOQ)、准确度、精密度和回收率测试，对分析方法进行了验证。验证标准是根据AOAC国际和日本验证指南设置的。各化合物的线性关系几乎为决定系数(R2)&0.98。所有受试抗生素的最低检测限量均为10μg/kg。结果表明，该方法能够对牛肉、猪肉和鸡肉中的36种、33种和37种化合物进行定量分析。该方法可用于三种肉类(猪肉、牛肉和鸡肉)中抗生素的快速、简便的多残留筛选。
This study involved the development of a multiresidue method for the rapid analysis of 43 antibiotics in meats using ultrahigh-performance liquid chromatography–tandem mass spectrometry. This method was performed using dispersive-solid phase extraction, which is able to analyze 20 samples within 2 h. All compounds were determined simultaneously on a C18 separation column with gradient elution. Validation of the analytical method was performed by carrying out linearity, limit of quantification (LOQ), accuracy, precision, and recovery tests in different meat products. The validation criteria were set according to AOAC International and Japanese validation guidelines. The linearity of each compound was almost the coefficient of determination (r2) > 0.98. The LOQs of all tested antibiotics were <10 μg/kg. The results verify that this method is capable of quantitative analysis of 36, 33, and 37 compounds in beef, pork, and chicken, respectively. This method can be used for rapid and easy multiresidue screening of antibiotics for three meats (pork, beef, and chicken).