Real-Time Monitoring of Cellular Bioenergetics with a Multianalyte Screen-Printed Electrode.
Real-Time Monitoring of Cellular Bioenergetics with a Multianalyte Screen-Printed Electrode.
复制标题
使用多分析物丝网印刷电极实时监测细胞生物能。
DOI：
10.1021/acs.analchem.5b01533
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发表时间：
2015
影响因子：
7.4
通讯作者：
Cliffel,DavidE
中科院分区：
文献类型：
--
作者：
McKenzie,JenniferR;Cognata,AndrewC;Davis,AnnaN;Wikswo,JohnP;Cliffel,DavidE
实时监测细胞生物能量学的变化可以为疾病和毒性的作用机制提供新的见解。这项工作描述了一种多分析物丝网印刷电极的开发，用于检测细胞生物能量学的分析物：葡萄糖，乳酸盐，氧气和pH值。铂丝网印刷电极是在内部设计的，并由松树研究仪器印刷。电化学电镀技术用于形成准参比电极和pH电极。使用Dimatix材料喷墨打印机来存款酶和聚合物膜以形成葡萄糖、乳酸盐和氧的传感器。这些传感器在本体溶液和微流体环境中进行了评估，发现它们的行为可重复，寿命长达6周。发现基于酶的传感器的线性范围和检测限与酶负载量成反比关系，并且发现氧化铱pH传感器具有超能斯特响应。进行了传感器封闭在具有RAW 264.7巨噬细胞的微流体通道内的初步测量，以证明传感器进行实时微生理测量的能力。
Real-time monitoring of changes to cellular bioenergetics can provide new insights into mechanisms of action for disease and toxicity. This work describes the development of a multianalyte screen-printed electrode for the detection of analytes central to cellular bioenergetics: glucose, lactate, oxygen, and pH. Platinum screen-printed electrodes were designed in-house and printed by Pine Research Instrumentation. Electrochemical plating techniques were used to form quasi-reference and pH electrodes. A Dimatix materials inkjet printer was used to deposit enzyme and polymer films to form sensors for glucose, lactate, and oxygen. These sensors were evaluated in bulk solution and microfluidic environments, and they were found to behave reproducibly and possess a lifetime of up to 6 weeks. Linear ranges and limits of detection for enzyme-based sensors were found to have an inverse relationship with enzyme loading, and iridium oxide pH sensors were found to have super-Nernstian responses. Preliminary measurements where the sensor was enclosed within a microfluidic channel with RAW 264.7 macrophages were performed to demonstrate the sensors’ capabilities for performing real-time microphysiometry measurements.