Intrachanges as part of complex chromosome-type exchange aberrations
Intrachanges as part of complex chromosome-type exchange aberrations
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DOI：
10.1016/s0027-5107(02)00078-7
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发表时间：
2002-07-25
影响因子：
2.3
通讯作者：
Natarajan, AT
中科院分区：
文献类型：
--
作者：
Boei, JJWA;Vermeulen, S;Natarajan, AT
在细胞周期的G(0)/G(1)期，电离辐射引起的染色体型交换畸变被认为是染色体断裂不合法重新连接的结果。从大量使用染色体绘画的研究中可以看出，即使在中等剂量的辐射之后，这些交换的很大一部分是复杂的。它们中的大多数是由三个或更多断裂末端之间的自由相互作用产生的。其他研究表明染色体在间期细胞核中占据不同的区域。由于离得很近的断裂具有增强的相互作用概率，因此在电离辐射后，许多相互作用的断裂似乎很可能出现在一条染色体或染色体臂内。不幸的是，即使采用复杂的分子细胞遗传学检测方法（如mFISH）将所有染色体对涂上不同的颜色，大多数这些内改变仍未被检测到。在本文中，我们评估了全彩绘画在检测复杂交换和正确解释断裂相互作用方面的局限性。(C) 2002 Elsevier Science B.V.版权所有
The chromosome-type exchange aberrations induced by ionizing radiation during the G(0)/G(1) phase of the cell cycle are believed to be the result of illegitimate rejoining of chromosome breaks. From numerous studies using chromosome painting, it has emerged that even after a moderate dose of radiation, a substantial fraction of these exchanges is complex. Most of them are derived from the free interaction between the ends of three or more breaks. Other studies have demonstrated that chromosomes occupy distinct territories in the interphase nucleus. Since breaks that are in close proximity have an enhanced interaction probability, it seems likely that after ionizing radiation many of the interacting breaks will be present within one chromosome or chromosome arm. Unfortunately, the majority of these intrachanges remain undetected, even when sophisticated molecular cytogenetic detection methods (i.e. mFISH) are applied to paint all chromosome pairs in distinct colors. In the present paper, we evaluate the limitations of full-color painting for the detection of complex exchanges and the correct interpretations of break interactions. (C) 2002 Elsevier Science B.V. All rights reserved.