Cobalt chloride improves angiogenic potential of CD133+ cells.
Cobalt chloride improves angiogenic potential of CD133+ cells.
复制标题
氯化钴可提高 CD133 细胞的血管生成潜力。
DOI：
10.2741/4048
复制
发表时间：
2012-06
期刊：
影响因子：
--
通讯作者：
昝涛
中科院分区：
文献类型：
--
作者：
昝涛
脐带血来源的CD133 +细胞具有分化为内皮细胞并诱导新血管生长的能力。缺氧诱导因子 - 1（HIF - 1）是缺氧或缺氧模拟剂反应的调节剂，可激活SDF - 1/C（最后这个“SDF - 1/C”不完整，可能影响准确理解）
Umbilical cord blood-derived CD133+ cells exhibit the ability to differentiate into endothelial cells and induce new blood vessel growth. Hypoxia-inducible factor-1 (HIF-1), a regulator of hypoxia or the hypoxia-mimetic agent response, actives the SDF-1/CXCR4 signaling pathway and thus plays an important role in angiogenesis in-vivo. In this study we aim to investigate whether CD133+ cells enhance angiogenic ability through hypoxia or CoCl2 in vitro. The CD133+ cells were cultured in normoxia (20 Percent O2), hypoxia (10 Percent O2, 3 Percent O2), or in various concentrations of CoCl2 (50 microM/L, 100 microM/L, 200 microM/L) and subjected to in vitro flow cytometric analysis, tubule formation, as well as migration and proliferation assays. The results demonstrate that both environmental hypoxia and CoCl2 induced hypoxia result in significantly increased CD133+ cell migration, proliferation, and tubule-like structure formation compared with normoxia culture conditions. The HIF-1a, SDF-1, and VEGF protein and gene expression level in conditions of hypoxia is higher than that found in normaxia conditions. Collectively, these data suggest that angiogenic potential of CD133+ cells is influenced by hypoxia or a hypoxia mimetic agent in vitro.