Expressed Sequence Tag Analysis of Vanadocytes in a Vanadium-Rich Ascidian, Ascidia sydneiensis samea
Expressed Sequence Tag Analysis of Vanadocytes in a Vanadium-Rich Ascidian, Ascidia sydneiensis samea
复制标题
富钒海鞘（Ascidia sydneiensis Samea）中钒细胞的表达序列标签分析
作者：
N. Yamaguchi;K. Kamino;T. Ueki;H. Michibata
海鞘科一些种类的钒的积累量超过350毫米，相当于海水中的107倍。在这些物种中，印章环细胞具有一个巨大的液泡，其中含有钒离子，其功能是积累钒的细胞，即钒细胞。为了研究这种现象的机制，我们对富钒海鞘细胞的互补DNA文库进行了表达序列标签(EST)分析。我们测定了1000个EST的核苷酸序列，并对SwissProt数据库进行了BLAST分析。我们发现了93个金属相关基因同源物的克隆，包括铁蛋白重亚单位、血蓝蛋白和金属硫蛋白。特别是，有两个EST与从紫云英血细胞中提取的低分子量钒结合蛋白的钒结合蛋白具有显著的相似性。我们已经将编码这些EST的基因命名为VaBin3和VaBin4。固定化金属离子亲和层析表明，这些新型的钒同系物与钒(IV)离子结合。
Some species in the family Ascidiidae accumulate vanadium at concentrations in excess of 350 mM, which corresponds to about 107 times higher than that in seawater. In these species signet ring cells, with a single huge vacuole in which vanadium ion is contained, function as vanadium-accumulating cells, vanadocytes. To investigate the mechanism underlying this phenomenon, we performed an expressed sequence tag (EST) analysis of a complementary DNA library from vanadocytes of a vanadium-rich ascidian, Ascidia sydneiensis samea. We determined the nucleotide sequences of 1000 ESTs and performed a BLAST analysis against the SwissProt database. We found 93 clones of metal-related gene homologues, including the ferritin heavy subunit, hemocyanin, and metallothionein. Two ESTs, in particular, exhibited significant similarity to vanabins that have been extracted from A. sydneiensis samea blood cells as low molecular weight vanadium-binding proteins. We have named the genes encoding these ESTs vanabin3 and vanabin4. Immobilized metal ion affinity chromatography revealed that these novel vanabin homologues bind vanadium(IV) ions.
影响因子：
3.9
作者：
Grady, JK;Shao, JL;Chasteen, ND
通讯作者：
Chasteen, ND