Replicating potato spindle tuber viroid RNA is accompanied by short RNA fragments that ave characteristic of post-transcriptional gene silencing
Replicating potato spindle tuber viroid RNA is accompanied by short RNA fragments that ave characteristic of post-transcriptional gene silencing
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DOI：
10.1093/nar/29.11.2395
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发表时间：
2001-06-01
影响因子：
14.9
通讯作者：
Tabler, M
中科院分区：
文献类型：
--
作者：
Papaefthimiou, I;Hamilton, AJ;Tabler, M
接种马铃薯纺锤体块茎类病毒(PSTVd)的番茄植株的低分子量组分含有一组PSTVd特异的短小RNA。主要成分为22和23个核苷酸的RNA，分别代表类病毒基因组的不同结构域。这种不同的RNA物种的出现表明，核复制的PSTVd RNA诱导转录后基因沉默。短RNA在接种后30天略多于后期，并且存在于感染了PSTVd的轻度、重度或致死性分离物的植株中。PSTVd特异小RNA的数量与类病毒分离物的毒力程度之间没有明显的相关性。
The low molecular weight fraction of tomato plants inoculated with potato spindle tuber viroid (PSTVd) contains a population of short PSTVd-specific RNAs of either polarity. The main constituents were RNAs of 22 and 23 nt representing different domains of the viroid genome. The occurrence of such distinct RNA species indicated that the nuclear replicating PSTVd RNA induces post-transcriptional gene silencing. The short RNAs were slightly more abundant at 30 days post-inoculation than at later stages and were present in plants infected with a mild, severe or lethal isolate of PSTVd. There was no apparent correlation between the quantity of small PSTVd-specific RNAs and the degree of virulence of the viroid isolate.