Sickle blood contains tissue factor-positive microparticles derived from endothelial cells and monocytes
Sickle blood contains tissue factor-positive microparticles derived from endothelial cells and monocytes
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DOI：
10.1182/blood-2003-03-0693
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发表时间：
2003-10-01
期刊：
影响因子：
20.3
通讯作者：
Hebbel, RP
中科院分区：
文献类型：
--
作者：
Shet, AS;Aras, O;Hebbel, RP
据报道，镰状细胞病（SCD）中的血液微粒（MP）仅来源于红细胞和血小板。然而，在SCD中，内皮细胞和单核细胞被激活并异常表达组织因子（TF）。因此，镰状血可能含有来自这些细胞的TF阳性Mps。使用流式细胞术计数和表征Mps，我们发现镰状细胞病受试者与对照受试者相比，在危象（P = .0001）和稳态（P = .02）时总Mps升高。这些Mps来源于红细胞、血小板、单核细胞和内皮细胞。与对照组相比，镰状危象组（P = .0001）和稳态组（P = .02）红细胞来源的Mps升高，单核细胞来源的Mps也升高（分别为P = .0004和P = .009）。与对照组相比，镰状危象患者的内皮和血小板源性MP升高。总TF阳性MP在镰状危象中高于稳态（P = 0.004）和对照组（P <0.0001），并且来源于单核细胞和内皮细胞。镰状MP缩短血浆凝固时间与控制MP相比，TF抗体部分抑制这种促凝血活性。与对照组相比，镰状细胞病患者的凝血标志物升高，并与总Mps和TF阳性Mps相关（两者均P <0.01）。这些数据支持SCD是单核细胞和内皮细胞活化以及TF活性异常的炎症状态的概念。(C)2003年，美国血液学会。
Blood microparticles (MPs) in sickle cell disease (SCD) are reportedly derived only from erythrocytes and platelets. Yet in SCD, endothelial cells and monocytes are activated and abnormally express tissue factor (TF). Thus, sickle blood might contain TF-positive Mps derived from these cells. With the use of flow cytometry to enumerate and characterize Mps, we found total Mps to be elevated in crisis (P = .0001) and steady state (P = .02) in subjects with sickle cell disease versus control subjects. These Mps were derived from erythrocytes, platelets, monocytes, and endothellal cells. Erythrocyte-derived Mps were elevated in sickle crisis (P = .0001) and steady state (P = .02) versus control subjects, as were monocytederived Mps (P = .0004 and P = .009, respectively). Endothelial and platelet-derived MPs were elevated in sickle crisis versus control subjects. Total TF-positive MPs were elevated in sickle crisis versus steady state (P = .004) and control subjects (P < .0001) and were derived from both monocytes and endothelial cells. Sickle MPs shortened plasma-clotting time compared with control Mps, and a TF antibody partially inhibited this procoagulant activity. Markers of coagulation were elevated in patients with sickle cell disease versus control subjects and correlated with total Mps and TF-positive Mps (P < .01 for both). These data support the concept that SCD is an inflammatory state with monocyte and endothelial activation and abnormal TF activity. (C) 2003 by The American Society of Hematology.