Alteration of the Cloudman melanoma cell cycle by prostaglandins E1 and E2 determined by using a 5-bromo-2'-deoxyuridine method of DNA analysis.
Alteration of the Cloudman melanoma cell cycle by prostaglandins E1 and E2 determined by using a 5-bromo-2'-deoxyuridine method of DNA analysis.
复制标题
使用 DNA 分析的 5-溴-2-脱氧尿苷方法测定前列腺素 E1 和 E2 对 Cloudman 黑色素瘤细胞周期的改变。
DOI：
10.1002/jcp.1041360206
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发表时间：
1988
影响因子：
5.6
通讯作者：
Nordlund,JJ
中科院分区：
文献类型：
--
作者：
Abdel-Malek,ZA;Swope,VB;Trinkle,LS;Ferroni,EN;Boissy,RE;Nordlund,JJ
Prostaglandins (PGs) E1and E2stimulate tyrosinase activity and suppress the proliferation of Cloudman S91 melanoma cells by altering their progression through the cell cycle. Prostaglandin E1and PGE2have prolonged or residual effects on melanoma cells. Cells treated for 5 or 24 hours with 10 μg/ml PGE2demonstrated decreased proliferation and increased tyrosinase activity for 48 hours after removal of the PGs. The effects of PGs on the cell cycle were investigated by determining total DNA content in cells stained with propidium iodide (Pl) and analyzed by a fluorescence activated cell sorter (FACS). Prostaglandin E1blocked cells in G2phase after 5 hours of treatment, corresponding to when inhibition of proliferation was first evident. Similarly, after 9 hours of treatment with PGE2, more cells were in late S, early G2phase and less in G1than their control counterparts. Also, melanoma cells were pulse‐labeled with 5‐bromo‐2′‐deoxyuridine (BrdUrd) prior to or at the end of PG treatment and then stained with a fluoresceinated monoclonal antibody to BrdUrd, and with Pl. This allows one to observe how BrdUrd‐labeled S‐phase cells cycle with time. Both PGE1and PGE2inhibit proliferation by blocking cells in G2phase of the cell cycle. The PG‐induced block in G2may be required by melanoma cells to synthesize mRNA and proteins that are essential for stimulation of tyrosinase activity. Ultrastructurally, only a subpopulation of the cells treated with PGE1or PGE2contained more mature melanosomes than control cells.
