Rgs1 and Gnai2 regulate the entrance of B lymphocytes into lymph nodes and B cell motility within lymph node follicles
Rgs1 and Gnai2 regulate the entrance of B lymphocytes into lymph nodes and B cell motility within lymph node follicles
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DOI：
10.1016/j.immuni.2005.01.017
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发表时间：
2005-03-01
期刊：
影响因子：
32.4
通讯作者：
Kehrl, JH
中科院分区：
文献类型：
--
作者：
Han, SB;Moratz, C;Kehrl, JH
G蛋白偶联受体与G α（i）偶联的信号传导有助于触发淋巴细胞进出淋巴结。在这里，我们表明，调节这些受体的信号输出显着改变B细胞贩运。野生型和Rgs 1（-/-）小鼠过继转移的B细胞的活体显微镜检查显示，Rgs 1（-/-）B细胞比野生型B细胞更好地粘附于淋巴结高内皮微静脉，更好地归巢于淋巴结，并且在淋巴结滤泡内移动更快。相比之下，来自Gnai 2（-/-）小鼠的B细胞进入淋巴结的能力较差，并且比野生型B细胞移动得更慢。Gnai 2（-/-）小鼠通常缺乏多个外周淋巴结，并且它们的B细胞对趋化因子的反应很差，表明G α（i1）和G α（i3）对G α（i2）的损失的补偿很差。这些结果证明了Rgs 1和Gnai 2在B细胞运输进入淋巴结和在淋巴结内的相反作用。
Signaling by G protein-coupled receptors coupled to G alpha(i) assists in triggering lymphocyte movement into and out of lymph nodes. Here, we show that modulating the signaling output from these receptors dramatically alters B cell trafficking. Intravital microscopy of adoptively transferred B cells from wild-type and Rgs1(-/-) mice revealed that Rgs1(-/-) B cells stick better to lymph node high endothelial venules, home better to lymph nodes, and move more rapidly within lymph node follicles than do wild-type B cells. In contrast, B cells from Gnai2(-/-) mice enter lymph nodes poorly and move more slowly than do wild-type B cells. The Gnai2(-/-) mice often lack multiple peripheral lymph nodes, and their B cells respond poorly to chemokines, indicating that G alpha(i1) and G alpha(i3) poorly compensate for the loss of G alpha(i2). These results demonstrate opposing roles for Rgs1 and Gnai2 in B cell trafficking into and within lymph nodes.