Protein Adsorption of Ultrafine Metal Oxide and Its Influence on Cytotoxicity toward Cultured Cells
Protein Adsorption of Ultrafine Metal Oxide and Its Influence on Cytotoxicity toward Cultured Cells
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DOI：
10.1021/tx800289z
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发表时间：
2009-03-01
影响因子：
4.1
通讯作者：
Yoshida, Yasukazu
中科院分区：
文献类型：
--
作者：
Horie, Masanori;Nishio, Keiko;Yoshida, Yasukazu
关于金属氧化物纳米颗粒的细胞反应的研究已有很多报道。然而，金属氧化物纳米颗粒对细胞的吸附能力的影响是未知的。本研究的目的是了解金属氧化物纳米颗粒吸附对体外细胞活力的影响。评价了NiO、ZnO、TiO 2、CeO 2、SiO 2和Fe 2 O 3等6种金属氧化物纳米粒子对添加有10%胎牛血清（DMEM-FBS）成分（血清蛋白和Ca 2+）的Dulbecco改良Eagle培养基的吸附能力。所有的金属氧化物纳米颗粒吸附蛋白质和Ca 2+的DMEM-FBS，特别是，TiO 2，CeO 2，和ZnO表现出较强的吸附能力。此外，检查了通过吸附到金属氧化物纳米颗粒而消耗培养基组分对细胞活力和增殖的影响。通过离心从分散体中除去颗粒，并将上清液施加到细胞上。培养上清对人角质形成细胞HaCaT和人肺癌细胞A549的细胞活力和增殖均有影响。特别是，细胞增殖强烈抑制的TiO 2和CeO 2分散体的上清液。上清液显示通过吸附到金属氧化物纳米颗粒的血清蛋白和Ca 2+的耗尽。当吸附效果被FBS预处理颗粒阻断时，抑制效果丧失。然而，在NiO和ZnO，这表明离子释放，预处理的抑制效果的减少没有显示。此外，在二氧化钛的初级颗粒尺寸和吸附能力的关联进行了检查。TiO 2的吸附能力取决于原生颗粒的大小。TiO 2纳米颗粒与蛋白质和Ca 2+的大小依赖性吸收，从而诱导细胞毒性。总之，金属氧化物纳米颗粒的吸附能力是评价低毒性材料体外细胞毒性的重要因素。
Many investigations about the cellular response by metal oxide nanoparticles in vitro have been reported. However, the influence of the adsorption ability of metal oxide nanoparticles toward cells is unknown. The aim of this study is to understand the influence of adsorption by metal oxide nanoparticles on the cell viability in vitro. The adsorption abilities of six kinds of metal oxide nanoparticles, namely, NiO, ZnO, TiO2, CeO2, SiO2, and Fe2O3, to Dulbecco's modified Eagle's medium supplemented with a 10% fetal bovine serum (DMEM-FBS) component such as serum proteins and Ca2+ were estimated. All of the metal oxide nanoparticles adsorbed proteins and Ca2+ in the DMEM-FBS; in particular, TiO2, CeO2, and ZnO showed strong adsorption abilities. Furthermore, the influence of the depletion of medium components by adsorption to metal oxide nanoparticles on cell viability and proliferation was examined. The particles were removed from the dispersion by centrifugation, and the supernatant was applied to the cells. Both the cell viability and the proliferation of human keratinocyte HaCaT cells and human lung carcinoma A549 cells were affected by the supernatant. In particular, cell proliferation was strongly inhibited by the supernatant of TiO2 and CeO2 dispersions. The supernatant showed depletion of serum proteins and Ca2+ by adsorption to metal oxide nanoparticles. When the adsorption effect was blocked by the pretreatment of particles with FBS, the inhibitory effect was lost. However, in NiO and ZnO, which showed ion release, a decrease of inhibitory effect by pretreatment was not shown. Furthermore, the association of the primary particle size and adsorption ability was examined in TiO2. The adsorption ability of TiO2 depended on the primary particle size. The TiO2 nanoparticles were size dependently absorbed with proteins and Ca2+, thereby inducing cytotoxicity. In conclusion, the adsorption ability of metal oxide nanoparticles is an important factor for the estimation of cytotoxicity in vitro for low-toxicity materials.