Thy 1 (theta) antigen on the surface of morphologically distinct brain cell types
Thy 1 (theta) antigen on the surface of morphologically distinct brain cell types
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Thy 1 (theta) 抗原位于形态不同的脑细胞类型表面
DOI：
10.1016/0092-8674(75)90114-2
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发表时间：
1975
期刊：
影响因子：
64.5
通讯作者：
E. Thompson
中科院分区：
文献类型：
--
作者：
R. Mirsky;E. Thompson
最初不表达Thy 1抗原的15-19日龄胎小鼠脑的表面培养物在培养的4 - 11天之间产生抗原。可通过培养板上两种形态不同类型细胞的活细胞的免疫荧光检测抗原。神经元样细胞和包括成纤维细胞在内的更一般类别的细胞都可以表达Thy 1抗原。该抗原在某些神经胶质类型的细胞上不表达。这种方法使人们能够同时在形态学和免疫学上区分培养中不同类型的脑细胞。
Surface cultures of 15-19 day old fetal mouse brain that do not initially express the Thy 1 antigen develop the antigen between 4 and 11 days in culture. The antigen can be detected by immunofluorescence of live cells on the culture plate on two morphologically distinct types of cells. Both neuronal-looking cells and a more general category of cells which includes fibroblastic cells can express the Thy 1 antigen. The antigen is not expressed on certain cells of the glial type. This method enables one to distinguish between different types of brain cells in culture both morphologically and immunologically at the same time.