Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects
Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects
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DOI：
10.3389/fpls.2019.01234
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发表时间：
2019-10-14
影响因子：
5.6
通讯作者：
Ezura, Hiroshi
中科院分区：
文献类型：
--
作者：
Damayanti, Farida;Lombardo, Fabien;Ezura, Hiroshi
许多植物microRNA已被证明通过整合内部和环境线索来调节发育过程。最近，拟南芥F-box蛋白HAWAIIAN SKIRT（HWS）基因已被描述为在miRNA生物合成中的作用。我们已经在正向遗传筛选中分离出一个在HWS的假定直系同源物中突变的番茄（Solanum lycopersicum）品系。我们发现，番茄hws-1突变体表现出减少小叶锯齿，小叶融合，一定程度的花器官融合，并改变miRNA水平，类似于原来的A。拟南芥hws-1突变体我们还描述了新的表型，如兼性单性结实，生育力下降和开花延迟。slhws-1的单性结实特性受温度的影响，在较温暖的环境中单性结实率较高。相反，slhws-1在较冷的环境中生长时能够产生种子。我们表明，在突变体中的种子产量的减少主要是由于有缺陷的男性功能和几个miRNA的水平增加，根据以前的HWS研究，占异常的叶和花的表型，以及改变开花和果实发育过程。这是首次在肉质果实植物中对HWS基因的研究，为该基因在果实发育中的功能提供了新的认识。
A number of plant microRNAs have been demonstrated to regulate developmental processes by integrating internal and environmental cues. Recently, the Arabidopsis thaliana F-box protein HAWAIIAN SKIRT (HWS) gene has been described for its role in miRNA biogenesis. We have isolated in a forward genetic screen a tomato (Solanum lycopersicum) line mutated in the putative ortholog of HWS. We show that the tomato hws-1 mutant exhibits reduction in leaflet serration, leaflet fusion, some degree of floral organ fusion, and alteration in miRNA levels, similarly to the original A. thaliana hws-1 mutant. We also describe novel phenotypes for hws such as facultative parthenocarpy, reduction in fertility and flowering delay. In slhws-1, the parthenocarpy trait is influenced by temperature, with higher parthenocarpy rate in warmer environmental conditions. Conversely, slhws-1 is able to produce seeds when grown in cooler environment. We show that the reduction in seed production in the mutant is mainly due to a defective male function and that the levels of several miRNAs are increased, in accordance with previous HWS studies, accounting for the abnormal leaf and floral phenotypes as well as the altered flowering and fruit development processes. This is the first study of HWS in fleshy fruit plant, providing new insights in the function of this gene in fruit development.