Manganese-Based Magnetic Layered Double Hydroxide Nanoparticle: A pH-Sensitive and Concurrently Enhanced T-1/T-2-Weighted Dual-Mode Magnetic Resonance Imaging Contrast Agent
Manganese-Based Magnetic Layered Double Hydroxide Nanoparticle: A pH-Sensitive and Concurrently Enhanced T-1/T-2-Weighted Dual-Mode Magnetic Resonance Imaging Contrast Agent
复制标题
锰基磁性层状双氢氧化物纳米粒子：一种pH敏感且同时增强的T-1/T-2加权双模磁共振成像造影剂
DOI：
10.1021/acsbiomaterials.8b01618
复制
发表时间：
2019
影响因子：
--
通讯作者：
Gu Zi
中科院分区：
文献类型：
--
作者：
Xie Wensheng;Guo Zhenhu;Cao Zhenbang;Gao Qin;Wang Dan;Boyer Cyrille;Kavallaris Maria;Sun Xiaodan;Wang Xiumei;Zhao Lingyun;Gu Zi
干扰效应和选择性不足是制约双模式磁共振成像（MRI）造影剂发展的瓶颈。为了解决这些挑战并克服单模成像对比度的限制，已经成功合成了新型MgMnAl-层状双氢氧化物@氧化铁纳米颗粒（MgMnAl-LDH@IO NP），作为用于肿瘤组织MRI的同时增强的双模造影剂，具有敏感的pH响应和高功效。氧化铁纳米颗粒在MgMnAl-LDH纳米颗粒表面的附着导致了局部磁场强度的增加，导致了T1和T2弛豫的同时增强。体外MRI结果表明，MgMnAl-LDH@IO NP在T1和T2加权成像中均能作为pH敏感的对比剂（r1，pH5.0时为5.67 mM-1 s-1，pH7.4时为1.98 mM-1 s-1;r2，pH5.0时为369.12 mM-1 s-1，pH7.4时为225.29 mM-1 s-1）。通过成纤维细胞毒性试验，证实了双模式造影剂的生物相容性。体内双模式MR成像显示，MgMnAl-LDH@IO NP对荷瘤小鼠肿瘤组织的T1和T2加权成像清晰。MgMnAl-LDH@IO NP的合成方法简单、生物相容性好、刺激响应灵敏、体外和体内T1/T2信号增强等特点，使其在生物医学和临床应用中具有巨大的潜力。
The interference effect and lack of selectivity are the bottlenecks for dual-mode magnetic resonance imaging (MRI) contrast agent development. To address these challenges and overcome the single mode imaging contrast limitations, a novel MgMnAl-layered double hydroxide@iron oxide nanoparticle (MgMnAl-LDH@IO NP) has been successfully synthesized as a concurrently enhanced dual-mode contrast agent for MRI of tumor tissues with sensitive pH response and high efficacy. The attachment of iron oxide nanoparticles on the surface of MgMnAl-LDH NPs led to the increased local magnetic field intensity, inducing the concurrent enhancement of bothT1andT2relaxivity. Thein vitroMRI demonstrated that the MgMnAl-LDH@IO NP could act as a pH-sensitive contrast agent for bothT1- andT2-weighted MR imaging (r1, 5.67 mM–1s–1under pH 5.0 and 1.98 mM–1s–1under pH 7.4;r2, 369.12 mM–1s–1under pH 5.0 and 225.29 mM–1s–1under pH 7.4). The biocompatibility of the dual-mode contrast agent was revealed by the cytotoxicity test on fibroblast cells. Furtherin vivodual-mode MR imaging exhibited that the MgMnAl-LDH@IO NP showed clearT1- andT2-weighted MR imaging of tumor tissues in breast-tumor-bearing mice. The facile synthetic method, desirable biocompatibility, sensitive stimuli response, and concurrently enhancedT1/T2MRI signals bothin vitroandin vivoencourage the great potential biomedical and clinical applications of MgMnAl-LDH@IO NP in MR imaging with improved accuracy.