Radioiodination of Parasite Antigens with 1,3,4,6,-Tetrachloro-3α,6α-diphenylglycoluril (IODOGEN): Studies with Zygotes of Plasmodium gallinaceum
Radioiodination of Parasite Antigens with 1,3,4,6,-Tetrachloro-3α,6α-diphenylglycoluril (IODOGEN): Studies with Zygotes of Plasmodium gallinaceum
复制标题
用 1,3,4,6,-四氯-3α,6α-二苯基甘脲 (IODOGEN) 对寄生虫抗原进行放射性碘化：鸡疟原虫合子的研究
DOI：
--
复制
发表时间：
1982
期刊：
影响因子：
--
通讯作者：
R. Carter
中科院分区：
文献类型：
--
作者：
R. J. Howard;D. Kaushal;R. Carter
用碘试剂1，3，4，6-四氯-3α，6α-二苯基甘脲(IODOGEN)标记鸡疟原虫受精卵的抗原，并与乳过氧化物酶催化的放射性碘标记作对照。IODOGEN法标记的蛋白质很可能位于受精卵的表面，因为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法分析的标记蛋白质的图谱与乳过氧化物酶标记的蛋白质图谱非常相似。此外，标记的蛋白质仅代表全部考马斯亮蓝染色蛋白质的子集。经IODOGEN或乳过氧化物酶标记后，核素标记的受精卵蛋白呈免疫反应。IODOGEN方法在技术上比乳过氧化物酶方法简单得多，不需要添加额外的蛋白质或过氧化氢。IODOGEN标记的优点，加上这两种方法获得的结果基本相同，使得IODOGEN方法对于标记寄生虫抗原具有普遍的吸引力。
The iodinating reagent 1,3,4,6-tetrachloro-3 alpha, 6 alpha-diphenylglycoluril (IODOGEN) was used to label antigens on zygotes of Plasmodium gallinaceum with parallel studies using lactoperoxidase-catalyzed radioiodination for comparison. Proteins labeled by the IODOGEN method are most probably on the surface of the zygote, as the pattern of labeled proteins analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was very similar to the pattern of lactoperoxidase-labeled proteins. Furthermore, the labeled proteins represented only a subset of the total Coomassie Blue-stained proteins. The radioiodinated zygote proteins were immunoreactive after IODOGEN or lactoperoxidase labeling. The IODOGEN method is technically much more simple than the lactoperoxidase method and does not require the addition of extraneous proteins or H2O2. The advantages of IODOGEN labeling, together with the essential equivalence of results obtained by these two methods, make the IODOGEN method attractive for labeling parasite antigens in general.