Degranulating stimuli decrease the neagative surface charge and increase the adhesiveness of human neutrophils.
Degranulating stimuli decrease the neagative surface charge and increase the adhesiveness of human neutrophils.
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脱粒刺激可减少表面负电荷并增加人类中性粒细胞的粘附性。
DOI：
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发表时间：
1980
影响因子：
15.9
通讯作者：
J. Gallin
中科院分区：
文献类型：
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作者：
J. Gallin
趋化因子减少中性粒细胞（多形核白细胞[PMN]）的负表面电荷，这被推测在体内PMN的聚集和聚集中很重要。溶酶体酶的脱颗粒也增强了PMN的粘附和聚集。为了进一步评估脱颗粒、表面电荷、粘附和聚集之间的可能关系，将人外周血PMN（通过Hypaque-Ficoll和葡聚糖沉淀法分离）暴露于促分泌物离子载体A23187、肉豆肉酸酯、豆豆蛋白A和趋化因子（部分纯化的C5a或合成的f-met-leu-phe）和细胞松弛素b。PMN与促分泌剂孵育后，PMN表面电荷比趋化因子孵育后降低的程度更大。f-met-leu-phe +细胞松弛素B诱导的表面电荷减少需要细胞外钙和镁。离子载体a23187诱导的表面电荷变化依赖于细胞外钙而不依赖于镁，而肉豆酸酯磷的作用仅部分依赖于Ca（++）和Mg（++）。促分泌剂引起的表面电荷变化与溶菌酶的释放量和细胞对塑料表面的粘附性增加有关。这些观察结果表明，溶酶体颗粒内容物的胞吐与中性粒细胞表面电荷的减少有关，并且表面电荷的减少与中性粒细胞聚集和粘附性的促进之间似乎存在相关性。然而，这些事件之间的因果关系尚未确立，这种关系可能只是暂时的。
Chemotactic factors decrease the negative surface charge of neutrophils (polymorphonuclear leukocytes [PMN]) and this has been speculated to be important in PMN margination and aggregation in vivo. PMN adherence and aggregation are also enhanced by degranulation of lysosomal enzymes. To further assess the possible relationship between degranulation, surface charge, adherence, and aggregation, human peripheral blood PMN (isolated by Hypaque-Ficoll and dextran sedimentation) were exposed to the secretagogues ionophore A23187, phorbol myristate acetate, concanavalin A, and chemotactic factors (partially purified C5a or the synthetic peptide f-met-leu-phe) plus cytochalasin B. Surface charge was measured in a cytopherometer. After incubation of PMN with secretagogues, PMN surface charge was decreased to a greater extent than incubation of PMN with chemotactic factors. The decreased surface charge induced by f-met-leu-phe plus cytochalasin B required both extracellular calcium and magnesium. The ionophore A23187-induced surface charge changes were dependent on extracellular calcium but not magnesium whereas the phorbol myristate acetate effect was only partially dependent on Ca(++) and Mg(++). The surface charge changes induced by secretagogues were related to both the amount of lysozyme released and to the increased adhesiveness of cells to plastic surfaces. These observations indicate exocytosis of lysosomal granule contents is associated with decreases in neutrophil surface charge, and there appears to be a correlation between decreases in surface charge and facilitation of neutrophil aggregation and adhesiveness. However, a causal relationship between these events has not been established, and the relationship may be simply temporal.