Demonstration that C-reactive protein decreases eNOS expression and bioactivity in human aortic endothelial cells
Demonstration that C-reactive protein decreases eNOS expression and bioactivity in human aortic endothelial cells
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DOI：
10.1161/01.cir.0000033116.22237.f9
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发表时间：
2002-09-17
期刊：
影响因子：
37.8
通讯作者：
Jialal, I
中科院分区：
文献类型：
--
作者：
Venugopal, SK;Devaraj, S;Jialal, I
背景 C 反应蛋白 (CRP) 是炎症的原型标志物，已被证明是心血管事件的独立预测因子。内皮一氧化氮合酶 (eNOS) 缺乏是动脉粥样硬化形成的关键事件。方法和结果 - 我们测试了 CRP 对培养的人主动脉内皮细胞 (HAEC) 中 eNOS 表达和生物活性的影响。 CRP 降低 HAEC 中的 eNOS mRNA、蛋白质丰度和酶活性。此外，CRP 显着降低了通过环 GMP 水平测定的 eNOS 生物活性。细胞与CRP预孵育也显着增加单核细胞对HAEC的粘附。结论-CRP导致HAEC中cNOS表达和生物活性的直接降低，进一步支持其在动脉粥样硬化形成中的作用。
Background-C-reactive protein (CRP), the prototypic marker of inflammation, has been shown to be an independent predictor of cardiovascular events. Endothelial nitric oxide synthase (eNOS) deficiency is a pivotal event in atherogenesis.Methods and Results-We tested the effect of CRP on eNOS expression and bioactivity in cultured human aortic endothelial cells (HAECs). CRP decreased eNOS mRNA, protein abundance, and enzyme activity in HAECs. Furthermore, eNOS bioactivity assayed by cyclic GMP levels was significantly reduced by CRP. Preincubation of cells with CRP also significantly increased the adhesion of monocytes to HAECs.Conclusion-CRP causes a direct reduction in cNOS expression and bioactivity in HAECs, further supporting its role in atherogenesis.