Detection of space radiation-induced double strand breaks as a track in cell nucleus
Detection of space radiation-induced double strand breaks as a track in cell nucleus
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DOI：
10.1016/j.bbrc.2009.09.114
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发表时间：
2009-12-18
影响因子：
3.1
通讯作者：
Ishioka, Noriaki
中科院分区：
文献类型：
--
作者：
Ohnishi, Takeo;Takahashi, Akihisa;Ishioka, Noriaki
为了确定高线性能量转移(LET)粒子等空间辐射对DNA的损伤，分析了在国际空间站冷冻箱中冷冻133天的人细胞中磷酸-H_2AX(伽马H_2AX)焦点的形成。将冰冻标本放回原地，培养30min后固定。在这里，我们通过免疫细胞化学方法显示了它们中的γ-H_2AX阳性灶的踪迹。结果表明，空间辐射，特别是高LET粒子，引起了DSB的径迹。根据核中径迹的形成，计算出相对较高能量的铁离子空间辐射(500 MeV/u，200keV/mum)的剂量率为0.7mSv/天。用CR-39型塑料核径迹探测器和热释光剂量计进行物理剂量测量，剂量率为0.5mSv/d。生物剂量学和物理剂量学的这些剂量率值相似。(C)2009 Elsevier Inc.保留所有权利。
To identify DNA damage induced by space radiations such as the high linear energy transfer (LET) particles, phospho-H2AX (gamma H2AX) foci formation was analyzed in human cells frozen in an International Space Station freezer for 133 days. After recovering the frozen sample to the earth, the cells were cultured for 30 min, and then fixed. Here we show a track of gamma H2AX positive foci in them by immuno-cytochemical methods. It is suggested that space radiations, especially high LET particles, induced DSBs as a track. From the formation of the tracks in nuclei, exposure dose rate was calculated to be 0.7 mSv per day as relatively high-energy space radiations of Fe-ions (500 MeV/u, 200 keV/mu m). From the physical dosimetry with CR-39 plastic nuclear track detectors and thermo-luminescent dosimeters, dose rate was 0.5 mSv per day. These values the exposed dose rate were similar between biological and physical dosimetries. (C) 2009 Elsevier Inc. All rights reserved.