Role of Atf1 and Pap1 in the induction of the catalase gene of fission yeast Schizosaccharomyces pombe
Role of Atf1 and Pap1 in the induction of the catalase gene of fission yeast Schizosaccharomyces pombe
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DOI：
10.1093/oxfordjournals.jbchem.a022599
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发表时间：
2000-02-01
影响因子：
2.7
通讯作者：
Mutoh, N
中科院分区：
文献类型：
--
作者：
Nakagawa, CW;Yamada, K;Mutoh, N
利用转录因子（Atf1和Pap1）突变体和ctt1（+）启动子区域的一系列缺失突变体，研究了分裂酵母裂糖酵母（Schizosaccharomyces pombe）在多种胁迫下过氧化氢酶基因（ctt1(+)）的诱导。转录因子Atf1及其结合位点是渗透胁迫、紫外线照射和热休克诱导ctt1（+）的必要条件。甲萘醌诱导产生超氧阴离子，所需元素A，区域从-111到-90（以转录起始位点编号！）+ 1)。氧化应激通过A元件诱导该基因的因子被确定为转录因子Pap1。我们还发现，在Pap1突变细胞中，Atf1被甲萘醌激活，尽管在Pap1（+）细胞中，它没有被甲萘醌激活。在多种胁迫下，pap1细胞的过氧化氢酶活性虽然受到mRNA的诱导，但并没有增加，这表明pap1在过氧化氢酶活性的表达中起一定的作用。
We examined the induction of the catalase gene (ctt1(+)) of fission yeast Schizosaccharomyces pombe in response to several stresses by using mutants of transcription factors (Atf1 and Pap1) and a series of deletion mutants of the ctt1(+) promoter region. A transcription factor, Atf1, and its binding site are necessary for the induction of ctt1(+) by osmotic stress, UV irradiation, and heat shock. Induction by menadione treatment, which produces superoxide anion, required element A, the region from -111 to -90 (numbered with the transcription start site! as +1). The factor responsible for the induction of the gene by oxidative stress via element A was identified as the transcription factor Pap1, We also found that Atf1 is activated by menadione treatment in pap1 mutant cells, although it is not activated by menadione treatment in pap1(+) cells. The activity of catalase is not increased in pap1 cells by several stresses, despite mRNA induction, suggesting that Pap1 plays some role in the expression of catalase activity.