Polysaccharide/Polynucleotide Complexes. Part 6
Polysaccharide/Polynucleotide Complexes. Part 6
复制标题
多糖/多核苷酸复合物。
DOI：
10.1002/cbdv.200490045
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发表时间：
2004
影响因子：
2.9
通讯作者：
S. Shinkai
中科院分区：
文献类型：
--
作者：
Kazuya Koumoto;M. Mizu;K. Sakurai;T. Kunitake;S. Shinkai
研究了由添加互补链或非互补链诱导的单链DNA/叶绿素兰三元复合物（ss-DNA β 2s-SPG）的光谱性质。将互补链添加到ss-DNA β 2s-SPG中会诱导结合的ss-DNA快速释放到互补链（DNA和RNA）中，而三元复合物在添加非互补链时不受影响。我们的实验表明，SPG具有基因载体不可缺少的络合性能。据我们所知，还没有关于利用这种非病毒基因载体的报道，这种载体可以实现结合的ss-DNA向互补链的智能释放。因此，我们认为，SPG，一种天然的中性多糖，具有成为新的ss-DNA载体的巨大潜力。
Spectroscopic properties of single‐stranded DNA/schizophyllan ternary complexes (ss‐DNA⋅2s‐SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss‐DNA⋅2s‐SPG induced the quick release of the bound ss‐DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss‐DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss‐DNA carrier.