Urogenital, maternal and neonatal isolates of Haemophilus influenzae: identification of unusually virulent serologically non-typable clone families and evidence for a new Haemophilus species.
Urogenital, maternal and neonatal isolates of Haemophilus influenzae: identification of unusually virulent serologically non-typable clone families and evidence for a new Haemophilus species.
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泌尿生殖系统、孕产妇和新生儿流感嗜血杆菌分离株：异常毒力血清学不可分型克隆家族的鉴定和新嗜血杆菌种的证据。
DOI：
10.1099/00221287-136-7-1203
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Musser,JM
中科院分区：
文献类型：
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作者：
Quentin,R;Goudeau,A;WallaceJr,RJ;Smith,AL;Selander,RK;Musser,JM
收集了117株流感嗜血杆菌，其中包括112株不能分型的菌株，这些菌株主要来自美国和法国的生殖器、产科和新生儿来源，其特征是10种代谢酶的凝胶迁移率。根据酶座位上的等位基因图谱，区分了86个不同的多位点染色体基因型(电泳型，Ets)。5个近缘IV型ETS的分离株与所有其他菌株高度分化，染色体DNA杂交显示，它们无疑代表了一个以前未被识别的嗜血杆菌物种。代表这些ETS的分离株主要是从产科感染和严重的新生儿疾病中恢复的，显然对生殖道具有特定的嗜性。这五种ETS菌株在美国和法国的样本中都存在，但只有在美国它们才会引起菌血症和脑膜炎，这可能反映了两国在患者管理方面的差异。虽然菌株被指定为Toh.从脑膜炎和/或败血症患者中分离到的69%的流感病毒分离株仅归属于两个克隆家系，这表明一些血清学上不能分型的H。起源于生殖道的流感病毒具有异乎寻常的毒性。
A collection of 117 strains ofHaemophilus influenzae, including 112 non-typable isolates recovered predominantly in the USA and France from genital, obstetric and neonatal sources, was characterized by the electrophoretic mobilities of 10 metabolic enzymes. Eighty-six distinctive multilocus chromosomal genotypes (electrophoretic types, ETs) were distinguished on the basis of allele profiles at the enzyme loci. Isolates of five allied biotype IV ETs were highly divergent from all other strains and hybridization of chromosomal DNA revealed that they undoubtedly represent a previously unrecognized species ofHaemophilus. Isolates representing these ETs were recovered predominantly from obstetric infections and serious neonatal diseases and apparently possess specific tropism for the genital tract. Strains of these five ETs were present in samples from both the USA and France, but only in the USA did they cause bacteraemia and meningitis, an occurrence which probably reflects differences in patient management between the two countries. Although strains assigned toH. influenzae(sensu stricto) were strongly polymorphic in multilocus enzyme genotype, 69% of isolates recovered from patients with meningitis and/or septicaemia were assigned to only two clone families, a result suggesting that some serologically non-typable strains ofH. influenzaeoriginating from the genital tract are unusually virulent.