AUTOANTIBODIES TO THE RIBOSOMAL P-PROTEINS REACT WITH A PLASMA MEMBRANE-RELATED TARGET ON HUMAN-CELLS
AUTOANTIBODIES TO THE RIBOSOMAL P-PROTEINS REACT WITH A PLASMA MEMBRANE-RELATED TARGET ON HUMAN-CELLS
复制标题
DOI：
10.1172/jci115707
复制
发表时间：
1992-04-01
影响因子：
15.9
通讯作者：
REICHLIN, M
中科院分区：
文献类型：
--
作者：
KOREN, E;REICHLIN, MW;REICHLIN, M
12-16% 的系统性红斑狼疮患者体内存在针对核糖体 P 蛋白的自身抗体，并且与神经精神疾病相关。 由于核糖体 P 蛋白位于细胞质中，其同源自身抗体的致病作用尚不清楚。 在本研究中，使用亲和纯化的抗 P 自身抗体来探索几种类型的人类和动物细胞的细胞表面。 免疫荧光和 EM 免疫金分析证明，在人肝癌细胞表面存在与 P 蛋白免疫显性羧基末端抗原相关的表位。 该表位的存在也被证明存在于人神经母细胞瘤细胞的表面上，并且在较小程度上存在于人成纤维细胞上。 此外，蛋白质印迹技术揭示了纯化的人和动物质膜中存在与核糖体 P0 蛋白密切相关或相同的 38 kD 蛋白。 细胞表面反应性 P 肽的可用性使得自身抗体能够直接影响表达该抗原靶标的细胞的功能和活力。 这描述了抗 P 抗体对疾病表达的可能影响之一。
Autoantibodies to ribosomal P-proteins are present in 12-16% of patients with systemic lupus erythematosus and are associated with neuropsychiatric disease. As the ribosomal P proteins are located in the cytoplasm, the pathogenic effects of their cognate autoantibodies are unclear. In this study affinity-purified anti-P autoantibodies were used to explore the cell surface of several types of human and animal cells. Immunofluorescence as well as EM immunogold analysis demonstrated, on the surface of human hepatoma cells, the presence of an epitope that is antigenically related to the immunodominant carboxy terminus of P-proteins. The presence of this epitope was also demonstrated on the surface of human neuroblastoma cells and, to a lesser extent, on human fibroblasts. Furthermore, the Western blot technique revealed in purified human and animal plasma membranes a 38-kD protein that is closely related or identical with ribosomal P0 protein. The availability of reactive P peptide on the surface of cells makes possible the direct effect of autoantibodies on the function and viability of cells that express this antigenic target. This delineates one of the possible impacts of anti-P antibodies in disease expression.