Confocal fluorescence imaging of DNA-functionalized carbon nanotubes
Confocal fluorescence imaging of DNA-functionalized carbon nanotubes
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DOI：
10.1021/nl025874t
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发表时间：
2003-02-01
期刊：
影响因子：
10.8
通讯作者：
Kappes, MM
中科院分区：
文献类型：
--
作者：
Hazani, M;Naaman, R;Kappes, MM
单壁碳纳米管（SWNT）与DNA通过碳酰亚胺辅助酰胺化共价修饰，产生一个高度水溶性的加合物。通过紫外可见光谱和共聚焦荧光显微镜研究了DNA与单壁碳纳米管之间的特异性和非特异性相互作用。单个束的荧光成像显示，SWNT-DNA加合物选择性地与互补链杂交，与非互补序列的非特异性相互作用最小。
Single-walled carbon nanotubes (SWNT) were covalently modified with DNA via carbodlimide-assisted amidation, yielding a highly watersoluble adduct. The specific and nonspecific interactions between DNA and SWNTs were examined by UV-vis spectroscopy and confocal fluorescence microscopy. Fluorescence imaging of individual bundles shows that the SWNT-DNA adducts hybridize selectively with complementary strands with minimal nonspecific interactions with noncomplementary sequences.