Two forms of parathyroid hormone-like adenylate cyclase-stimulating protein derived from tumors associated with humoral hypercalcemia of malignancy.
Two forms of parathyroid hormone-like adenylate cyclase-stimulating protein derived from tumors associated with humoral hypercalcemia of malignancy.
复制标题
两种形式的甲状旁腺激素样腺苷酸环化酶刺激蛋白，源自与恶性肿瘤体液高钙血症相关的肿瘤。
DOI：
10.1002/jbmr.5650020616
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Broadus,AE
中科院分区：
文献类型：
--
作者：
Stewart,AF;Burtis,WJ;Wu,T;Goumas,D;Broadus,AE
与体液高钙血症相关的恶性肿瘤(HHM)含有甲状旁腺激素样腺苷环化酶刺激蛋白(HACSPs)。我们之前从HHM相关的乳腺癌中纯化了17,000 mW的hACSP。这份报告描述了另外三个HHM相关肿瘤的hACSP的特征：两个典型的HHM相关肿瘤(鳞癌)和第三个罕见的肿瘤类型(嗜铬细胞瘤)。每个肿瘤在酸-尿素/乙醇-氯化钠中提取，并通过反相和大小排除高效液相色谱和等电聚焦检测腺苷环化酶刺激活性(ACSA)。每个肿瘤都含有一种高分子形式的ACSA，在三种分离程序中每一种都与17,000种分子质量的乳腺癌hACSP共洗脱。每一种还含有较低分子量形式(S)的ACSA(6,000-9,000分子量)。Nle8，18Tyr34bPTH(3-34)酰胺对这两种形式均有抑制作用。一些HHM相关肿瘤含有两种形式的hACSP，一种相对分子质量为17,000，另一种相对分子质量为6,000-9,000，这似乎是较大蛋白质的氨基末端裂解产物。
Tumors associated with humoral hypercalcemia of malignancy (HHM) contain parathyroid hormone‐like adenylate cyclase‐stimulating proteins (hACSPs). We previously purified a 17,000 MW hACSP from an HHM‐associated breast carcinoma. This report describes the characterization of hACSPs from three additional HHM‐associated tumors: two typical HHM‐associated tumors (squamous carcinomas) and a third unusual tumor type (pheochromocytoma).Each tumor was extracted in acid‐urea/ethanol‐sodium chloride, and adenylate cyclase‐stimulating activity (ACSA) was examined following reverse‐phase and size‐exclusion HPLC and isoelectric focusing. Each tumor contained a high molecular weight form of ACSA which co‐eluted with the 17,000 molecular weight breast carcinoma hACSP in each of the three separation procedures. Each also contained a lower molecular weight form(s) of ACSA (6,000–9,000 molecular weight). Both forms were inhibited by Nle8,18Tyr34bPTH(3–34) amide. The high molecular weight form was not changed to the lower molecular weight form by a reducing agent.Some HHM‐associated tumors contain two forms of hACSP, one with a molecular weight of 17,000 and another with a molecular weight of 6,000–9,000, which appears to be an amino‐terminal cleavage product of the larger protein.