Antioxidant nutrients and chronic disease: Use of biomarkers of exposure and oxidative stress status in epidemiologic research
Antioxidant nutrients and chronic disease: Use of biomarkers of exposure and oxidative stress status in epidemiologic research
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DOI：
10.1093/jn/133.3.933s
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发表时间：
2003-03-01
影响因子：
4.2
通讯作者：
Mayne, ST
中科院分区：
文献类型：
--
作者：
Mayne, ST
脂质、核酸或蛋白质的氧化已被认为涉及几种慢性疾病的病因学，包括癌症、心血管疾病、白内障、年龄相关性黄斑变性和一般的衰老。大量的研究已经调查了抗氧化营养素在预防这些和其他慢性疾病中的潜在作用。本文综述了以下抗氧化营养素：β-胡萝卜素和其他类胡萝卜素，维生素E，维生素C和硒。审查的第一部分强调了这些营养素暴露的生物标志物的效用以及与膳食摄入数据的关系。第二部分考虑人体氧化应激状态的功能测定，包括流行病学研究中使用的各种测定的优势和局限性。涵盖了体内和体外的各种功能测定，包括脂质氧化（硫代巴比妥酸反应物质，呼出的戊烷/乙烷，低密度脂蛋白抗氧化性，异前列烷），DNA氧化（氧化DNA碱基，如8-OHdG，氧化DNA的自身抗体，改良彗星测定）和蛋白质氧化（蛋白质羰基）的各种措施。为了说明的目的，研究了抗氧化营养素对这些功能标志物的影响。审查的结论与方法的问题和挑战的研究，涉及生物标志物暴露于抗氧化营养素和氧化应激状态的讨论。
Oxidation of lipid, nucleic acids or protein has been suggested to be involved in the etiology of several chronic diseases including cancer, cardiovascular disease, cataract, age-related macular degeneration and aging in general. A large body of research has investigated the potential role of antioxidant nutrients in the prevention of these and other chronic diseases. This review concentrates on the following antioxidant nutrients: beta-carotene and other carotenoids, vitamin E, vitamin C and selenium. The first part of the review emphasizes the utility of biological markers of exposure for these nutrients and the relationship to dietary intake data. The second part considers functional assays of oxidative stress status in humans including the strengths and limitations of various assays available for use in epidemiologic research. A wide variety of functional assays both in vivo and ex vivo, are covered, including various measures of lipid oxidation (thiobarbituric acid reactive substances, exhaled pentane/ethane, low-density lipoprotein resistance to oxidation, isoprostanes), DNA oxidation (oxidized DNA bases such as 8-OHdG, autoantibodies to oxidized DNA, modified Comet assay) and protein oxidation (protein carbonyls). Studies that have examined the effects of antioxidant nutrients on these functional markers are included for illustrative purposes. The review concludes with a discussion of methodologic issues and challenges for studies involving biomarkers of exposure to antioxidant nutrients and of oxidative stress status.