STK39, overexpressed in osteosarcoma, regulates osteosarcoma cell invasion and proliferation
STK39, overexpressed in osteosarcoma, regulates osteosarcoma cell invasion and proliferation
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DOI：
10.3892/ol.2017.6728
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发表时间：
2017-10-01
期刊：
影响因子：
2.9
通讯作者：
Hang, Dong-Hua
中科院分区：
文献类型：
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作者：
Huang, Tao;Zhou, Yuan;Hang, Dong-Hua
丝氨酸/苏氨酸激酶39（Serine/threonine kinase 39，STK 39）与高血压、孤独症、帕金森病及多种癌症的发生发展密切相关。本研究使用qPCR和western blot分析研究了STK 39在骨肉瘤中的表达和可能的作用。与正常骨组织相比，骨肉瘤中STK 39的mRNA和蛋白表达均上调。利用小干扰RNA转染，STK 39敲低到两个骨肉瘤细胞系，U2 OS和MG 63，并检查对细胞功能的影响。结果表明，STK 39下调抑制骨肉瘤细胞的增殖和侵袭。此外，STK 39在骨肉瘤细胞中的敲除显著影响与细胞增殖（增殖细胞核抗原和p21）和侵袭相关的蛋白质[Twist 1、基质金属蛋白酶（MMP）2和MMP 9]的表达。STK 39敲低降低了Smad 2/3的磷酸化。总之，我们的数据提供的证据表明，STK 39在骨肉瘤中过表达。STK 39可能是一个癌基因，通过调节骨肉瘤细胞的增殖和侵袭。
Serine/ threonine kinase 39 (STK39) is associated with hypertension, autism, Parkinson's disease and various types of cancer in recent years. This study investigated STK39 expression and possible roles in osteosarcoma using qPCR and western blot analysis. Compared to normal bone tissues, the mRNA and protein expression of STK39 was found to be upregulated in osteosarcoma. Using small interfering RNA transfection, STK39 was knocked down into two cell lines of osteosarcoma, U2OS and MG63, and the effects exerted on cell functioning were examined. The results showed that STK39 downregulation inhibited ostesarcoma cell proliferation and invasion. Moreover, STK39 knockdown in osteosarcoma cells significantly affected the expression of proteins connected to cell proliferation (proliferating cell nuclear antigen and p21) and invasion [Twist1, matrix metalloproteinase (MMP) 2 and MMP9]. Phosphorylation of Smad2/3 was reduced by STK39 knock down. In conclusion, our data provide evidence that STK39 was overexpressed in osteosarcoma. STK39 may serve as an oncogene by adjusting the proliferation and invasion of osteosarcoma cells.