DICTYOSTELIUM AMEBAS THAT LACK G-ACTIN-SEQUESTERING PROFILINS SHOW DEFECTS IN F-ACTIN CONTENT, CYTOKINESIS, AND DEVELOPMENT
DICTYOSTELIUM AMEBAS THAT LACK G-ACTIN-SEQUESTERING PROFILINS SHOW DEFECTS IN F-ACTIN CONTENT, CYTOKINESIS, AND DEVELOPMENT
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DOI：
10.1016/0092-8674(94)90199-6
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发表时间：
1994-10-21
期刊：
影响因子：
64.5
通讯作者：
SCHLEICHER, M
中科院分区：
文献类型：
--
作者：
HAUGWITZ, M;NOEGEL, AA;SCHLEICHER, M
为了研究普遍存在的肌动蛋白结合蛋白profilin在体内的功能，我们通过反义和基因干扰技术产生了缺乏一种或两种profilin异构体的网柄金藻突变体。虽然单个突变体的表型基本没有变化，但双突变体的行为发生了巨大的变化。细胞活力显著降低，单细胞体积比野生型细胞大10倍以上，在质膜下可见一条宽边的丝状肌动蛋白，丝状肌动蛋白浓度增加约60%-70%，子实体形成前发育受阻。此外，双突变株在正常条件下不能在摇瓶培养中生长，反映出胞质分裂受损。通过重新引入功能性Profilin I或Profilin II基因，可以挽救这种异常的表型。这项研究的数据表明，Profilin主要作为肌动蛋白隔离蛋白在Dictyostelialamebae中发挥作用。
To study in vivo functions of the ubiquitous actin-binding protein profilin, we generated by antisense and gene disruption techniques Dictyostelium mutants that lack one or both of the profilin isoforms. Whereas the single mutants showed an essentially unchanged phenotype, the behavior of the double mutant was drastically altered. Motility was significantly reduced, single cells were up to 10 times larger than wild-type cells and showed a broad rim of filamentous actin below the plasma membrane, the filamentous actin concentration was increased by about 60%-70%, and development was blocked prior to fruiting body formation. Furthermore, double mutants could not be grown in shaking culture under normal conditions, reflecting an impaired cytokinesis. The aberrant phenotype could be rescued by reintroducing a functional profilin I or profilin II gene. The data in this study suggest that profilin functions in Dictyostelium amoebae primarily as an actin-sequestering protein.