Juvenile hormone (JH) esterase activity but not JH epoxide hydrolase activity is downregulated in larval Adoxophyes honmai following nucleopolyhedroviruses infection
Juvenile hormone (JH) esterase activity but not JH epoxide hydrolase activity is downregulated in larval Adoxophyes honmai following nucleopolyhedroviruses infection
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核多角体病毒感染后，本米幼虫中保幼激素 (JH) 酯酶活性下调，但 JH 环氧化物水解酶活性不下调
DOI：
10.1016/j.jinsphys.2015.02.005
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发表时间：
2015
影响因子：
2.2
通讯作者：
Madoka Nakai
中科院分区：
文献类型：
--
作者：
Yasumasa Saito;Shizuo G. Kamita;Bruce D. Hammock;Yasuhisa Kunimi;Maki N. Inoue;Madoka Nakai
保幼激素和蜕皮激素是重要的昆虫发育激素。JH酯酶（JHE）和JH环氧化物水解酶（JHEH）是JH选择性酶，代谢JH，从而调节JH的滴度。已知杆状病毒可改变宿主的内分泌调节。已知核多角体病毒AdhoNPV和AdorNPV分别对日本卷蛾（鳞翅目：卷蛾科）具有缓慢和快速的杀伤活性。在这里，我们发现，当倒数第二（第四）instarA。用AdhoNPV和AdorNPV接种日本血吸虫，平均存活时间分别为9.7天和8.2天。幼虫蜕皮一次，但没有化蛹。AdhoNPV或AdorNPV感染的幼虫没有显示出JHE活性的显著增加，而在模拟感染的幼虫中发现，相反，它们显示出JHE活性的显著降低。相反，两种病毒感染对JHEH活性没有影响。为了进一步研究JHE的活性，我们克隆了日本曲霉（A.honmai，ahjhe）的JHE编码序列，并证实其编码具有生物活性的JHE。实时荧光定量PCR分析hjhe基因在4龄和5龄本氏按蚊中的表达，结果表明AdhoNPV和AdorNPV能够降低hjhe基因的表达水平。
Juvenile hormones (JHs) and ecdysteroids are critical insect developmental hormones. JH esterase (JHE) and JH epoxide hydrolase (JHEH) are JH-selective enzymes that metabolize JH and thus regulate the titer of JH. Baculoviruses are known to alter host endocrine regulation. The nucleopolyhedroviruses, AdhoNPV and AdorNPV, are known to have slow and fast killing activity againstAdoxophyes honmai(Lepidoptera: Tortricidae), respectively. Here we found that when penultimate (4th) instarA. honmaiare inoculated with AdhoNPV or AdorNPV, the mean survival time is 9.7 and 8.2 days, respectively. The larvae molted once but did not pupate. The AdhoNPV- or AdorNPV-infected larvae did not show a dramatic increase in JHE activity as was found in mock-infected larvae, instead they showed a marked decrease in JHE activity. In contrast, both viral infections had no effect on JHEH activity. In order to further characterize the JHE activity, the JHE-coding sequence ofA.honmai(ahjhe) was cloned and confirmed to encode a biologically active JHE. Quantitative real-time PCR analysis ofahjheexpression in 4th and 5th instarA.honmairevealed that AdhoNPV and AdorNPV are able to reduceahjheexpression levels.