Anomalous transport kinetics and the glucose carrier hypothesis.
Anomalous transport kinetics and the glucose carrier hypothesis.
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异常运输动力学和葡萄糖载体假说。
DOI：
10.1016/0005-2736(74)90320-4
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发表时间：
1974
期刊：
影响因子：
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通讯作者：
H. L. Tarpley
中科院分区：
文献类型：
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作者：
D. Regen;H. L. Tarpley
近年来，在人红细胞中葡萄糖转运的一些观察结果已经很难用载体模型来解释。三种新的模型被描述为替代载体假设的候选者。本文讨论了米勒（1968）生物物理学的一些反常发现。J. 8，1329-1338）和Stein的小组（（1972）Biochim. Biophys. Acta 266，161-173;（1966）Biochim. Biophys. Acta 127，179-193）在具有和不具有未搅拌层的载体模型方面进行了讨论（未搅拌层是载体和池内外的本体水相之间的扩散屏障）。没有简化假设的运营商模型（Regen，D. M. Morgan，H. E.（1964）Biochim. Biophys. Acta 79，151-166）加上米勒建议的未搅拌层，几乎容纳了所有被认为是拒绝的理由的数据。所有的葡萄糖和半乳糖的传输常数进行评估，包括在未搅拌层的扩散常数。描述了一些解释动力学数据的新方法。Geck描述的载体的不对称特征得到证实（（1971）Biochim. Biophys. Acta 241，462-472）。
In recent years several observations of glucose transport in human erythrocytes have been difficult to explain by the carrier model. Three new models have been described as candidates to supplant the carrier hypothesis. In this paper, some of the anomalous findings of Miller ((1968) Biophys. J. 8, 1329–1338) and of Stein's group ((1972) Biochim. Biophys. Acta 266, 161–173; (1966) Biochim. Biophys. Acta 127, 179–193) are discussed in terms of the carrier model with and without unstirred layers (unstirred layers being diffusion barriers between the carrier and the bulk aqueous phases inside and outside the cell). The carrier model with no simplifying assumptions (Regen, D. M. and Morgan, H. E. (1964) Biochim. Biophys. Acta 79, 151–166) with the addition of unstirred layers suggested by Miller, accommodates virtually all the data which have been considered grounds for its rejection. All transport constants for glucose and galactose are evaluated, including the diffusion constants in the unstirred layers. Some novel ways of interpreting kinetics data are described. The asymmetric features of the carrier described by Geck are confirmed ((1971) Biochim. Biophys. Acta 241, 462–472).