Molecular characterization and functional analysis of Cashmere goat mammalian target of rapamycin.
Molecular characterization and functional analysis of Cashmere goat mammalian target of rapamycin.
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DOI：
10.1089/dna.2011.1393
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发表时间：
2012-05
影响因子：
3.1
通讯作者：
Yan Liang;W. Bao;Chanchan Bao;Xiumei Miao;Hui-fang Hao;Shu-yu Li;Zhi-gang Wang;Dongjun Liu
中科院分区：
文献类型：
--
作者：
Yan Liang;W. Bao;Chanchan Bao;Xiumei Miao;Hui-fang Hao;Shu-yu Li;Zhi-gang Wang;Dongjun Liu
哺乳动物雷帕霉素靶蛋白（mTOR）是一种进化上保守的蛋白激酶，属于磷脂酰肌醇激酶相关激酶家族。我们描述了我们的mTOR的分子特征及其功能（GenBank登录号HM 114224）在绒山羊（Capra hircus）。山羊mTOR互补DNA为8617 bp，包括7650 bp的开放阅读框-对应于2549个氨基酸的多肽-和909 bp的3'非翻译区，其具有polyA区和在核苷酸8575-8580处的聚腺苷酸化信号。在生物信息学分析中，山羊mTOR具有典型的活性位点和结构域。采用实时荧光定量PCR法检测脑、心脏、睾丸、肝、脾、肾、肺组织中mTOR mRNA的表达，采用Western blot法检测胎儿成纤维细胞中mTOR的表达。胎儿成纤维细胞的活力在用CCI-779（mTOR的特异性抑制剂）处理时被抑制。结果表明，mTOR在绒山羊的7种组织中均有转录，在胎儿成纤维细胞中有翻译。胎儿成纤维细胞的增殖在mTOR的抑制下降低。
The mammalian target of rapamycin (mTOR) is an evolutionarily conserved protein kinase that belongs to the phosphatidylinositol kinase-related kinase family. We describe our molecular characterization of mTOR and its function (GenBank accession HM114224) in Cashmere goat (Capra hircus). The goat mTOR complementary DNA is 8617 bp, comprising an open reading frame of 7650 bp--corresponding to a polypeptide of 2549 amino acids--and a 909 bp 3' untranslated region with a polyA tract and a polyadenylation signal at nucleotides 8575-8580. In a bioinformatics analysis, goat mTOR has typical sites of activity and domains. mTOR mRNA was measured in brain, heart, testis, liver, spleen, kidney, and lung by real-time polymerase chain reaction, and the expression of mTOR in fetal fibroblasts was detected by western blot. The viability of fetal fibroblasts was inhibited on treatment with CCI-779, a specific inhibitor of mTOR. Our data supplied evidence that the transcription of mTOR was detected in the seven tissues in Cashmere goat, and mTOR protein was translated in fetal fibroblasts. The proliferation of fetal fibroblasts decreases on inhibition of mTOR.