Immunoadjuvants enhance the febrile responses of rats to endogenous pyrogen.
Immunoadjuvants enhance the febrile responses of rats to endogenous pyrogen.
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免疫佐剂增强大鼠对内源性热原的发热反应。
DOI：
10.1152/jappl.1989.67.5.1734
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Shimada,SG
中科院分区：
文献类型：
--
作者：
Stitt,JT;Shimada,SG
通过建立发热剂量反应曲线来表征雄性斯普拉格-道利大鼠对由人单核细胞产生的半纯化内源性热原的发热反应。然后给动物静脉注射多种物质，这些物质具有刺激网状内皮系统细胞吞噬活性和充当免疫佐剂的共同特性。使用的物质是酵母聚糖、脂多糖内毒素和胞壁酰二肽。注射任何这些免疫佐剂三天后，重新测量大鼠的发烧敏感性。在每种情况下，发烧剂量反应曲线的斜率都增加了三倍，并且在某些情况下，发烧反应的反应阈值降低了三到八倍。此外，免疫佐剂治疗后，内源性热原产生的体温最大升高增加了一倍以上。相比之下，乳胶珠也被网状内皮系统的细胞吞噬，但随后不会增加其吞噬指数，也不会增强免疫反应，因此对大鼠对内源性热原的发烧敏感性没有影响。根据这些发现，表明大鼠对内源性热原的发热反应是由具有网状内皮细胞的某些特性的细胞以某种方式介导的。这些假定细胞的位置必须靠近循环系统，因为本研究中使用的免疫佐剂大部分是大分子量分子，无法轻易穿过血脑屏障。
The febrile responses of male Sprague-Dawley rats to a semipurified endogenous pyrogen produced from human monocytes were characterized by establishing fever dose-response curves. The animals were then injected intravenously with a number of substances that possessed the common properties of stimulating the phagocytic activity of the cells of the reticuloendothelial system and of acting as immunoadjuvants. The substances used were zymosan, lipopolysaccharide endotoxin, and muramyl dipeptide. Three days after any of these immunoadjuvants were injected, the fever sensitivity of the rats was remeasured. In each case, the slope of the fever dose-response curve tripled, and in some instances the response threshold for fever response was reduced by factors of three to eight. Furthermore, the maximum increase in body temperature produced by the endogenous pyrogen was more than doubled after immunoadjuvant treatment. By contrast latex beads, which are also phagocytized by the cells of the reticuloendothelial system but do not subsequently increase their phagocytic index nor do they enhance immune responses, had no effect on the fever sensitivity of rats in response to endogenous pyrogen. In the light of these findings, it is suggested that the febrile responses of rats to endogenous pyrogen are mediated in some manner by cells that possess some of the properties of reticuloendothelial cells. The location of these putative cells must be close to the circulation, because the immunoadjuvants used in this study were, for the most part, large molecular weight molecules that could not cross the blood-brain barrier easily.