Pseudomonas aeruginosa induces pigment production and enhances virulence in a white phenotypic variant of Staphylococcus aureus.
Pseudomonas aeruginosa induces pigment production and enhances virulence in a white phenotypic variant of Staphylococcus aureus.
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DOI：
10.2147/idr.s49039
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发表时间：
2013-01-01
影响因子：
3.9
通讯作者：
Alavi, Mohammad
中科院分区：
文献类型：
--
作者：
Antonic, Vlado;Stojadinovic, Alexander;Alavi, Mohammad
葡萄球菌黄素是一种在应激条件下保护金黄色葡萄球菌的毒力因子。我们分离出两个色素变异体的S。金黄色葡萄球菌和一株铜绿假单胞菌来自单一伤口感染。S.金黄色葡萄球菌变体显示白色和黄色菌落表型。葡萄黄素合成的操纵子序列表明，编码和启动子区是相同的两个色素变体之间。群体感应控制某些细菌的色素合成。铜绿假单胞菌的群体感应分子也影响S。aureus转录。我们探讨了是否共同感染铜绿假单胞菌可以影响色素生产的白色S。金黄色变种在白色变体与选定数量的革兰氏阳性和革兰氏阴性细菌之间的共培养实验中，仅铜绿假单胞菌诱导白色变体中的色素产生。白色变体的基因表达分析未表明crtM和已知参与色素产生的其他基因（sigB、sarA、法尼基焦磷酸合酶基因[FPP-合酶]、hfq）的上调。相反，过氧化氢酶基因的转录显着上调后，共培养。铜绿假单胞菌诱导的色素合成和过氧化氢酶上调与多粘菌素B、过氧化氢和巨噬细胞细胞内环境的抗性增加相关。我们的数据表明，在一个白色表型变异的S.金黄色葡萄球菌，其通过种间通讯被共感染的铜绿假单胞菌激活。另一个S。金黄色葡萄球菌毒力因子，过氧化氢酶也被这种共感染细菌诱导。由此产生的表型变化与白色变体对应激条件的抗性直接相关。
Staphyloxanthin is a virulence factor which protects Staphylococcus aureus in stress conditions. We isolated two pigment variants of S. aureus and one strain of Pseudomonas aeruginosa from a single wound infection. S. aureus variants displayed white and yellow colony phenotypes. The sequence of the operons for staphyloxanthin synthesis indicated that coding and promoter regions were identical between the two pigment variants. Quorum sensing controls pigment synthesis in some bacteria. It is also shown that P. aeruginosa quorum-sensing molecules affect S. aureus transcription. We explored whether the co-infecting P. aeruginosa can affect pigment production in the white S. aureus variant. In co-culture experiments between the white variants and a selected number of Gram-positive and Gram-negative bacteria, only P. aeruginosa induced pigment production in the white variant. Gene expression analysis of the white variant did not indicate upregulation of the crtM and other genes known to be involved in pigment production (sigB, sarA, farnesyl pyrophosphate synthase gene [FPP-synthase], hfq). In contrast, transcription of the catalase gene was significantly upregulated after co-culture. P. aeruginosa-induced pigment synthesis and catalase upregulation correlated with increased resistance to polymyxin B, hydrogen peroxide, and the intracellular environment of macrophages. Our data indicate the presence of silent but functional staphyloxanthin synthesis machinery in a white phenotypic variant of S. aureus which is activated by a co-infecting P. aeruginosa via inter-species communication. Another S. aureus virulence factor, catalase is also induced by this co-infecting bacterium. The resulting phenotypic changes are directly correlated with resistance of the white variant to stressful conditions.