THE STRUCTURAL SPECIFICITY OF THE HIGH AFFINITY UPTAKE OF l‐GLUTAMATE AND l‐ASPARTATE BY RAT BRAIN SLICES
THE STRUCTURAL SPECIFICITY OF THE HIGH AFFINITY UPTAKE OF l‐GLUTAMATE AND l‐ASPARTATE BY RAT BRAIN SLICES
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大鼠脑切片高亲和力摄取 L-谷氨酸和 L-天冬氨酸的结构特异性
DOI：
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发表时间：
1972
影响因子：
4.7
通讯作者：
G. Johnston
中科院分区：
文献类型：
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作者：
V. Balcar;G. Johnston
摘要-大鼠大脑皮层对l -谷氨酸和l -天冬氨酸的高亲和力摄取系统可能不是针对这些可能的兴奋性突触递质所特有的，因为通过大鼠脑片观察到，苏- 3 -羟基- dl -天冬氨酸、l -半胱氨酸磺酸盐、l -半胱氨酸和d -天冬氨酸强烈抑制了对l - [3H]谷氨酸的高亲和力摄取，其方式与线性竞争抑制一致。因此，这些物质应被视为运输系统可能的底物。这四种酸性氨基酸中的每一种都以与谷氨酸引起的兴奋相似的方式激发中枢神经元，并且由于每种氨基酸都可能发生在脑组织中，因此应该研究它们可能在突触中的作用。
Abstract— The high affinity uptake system for l‐glutamate and l‐aspartate in rat cerebral cortex may not be specific for these likely excitatory synaptic transmitters, as threo‐3‐hydroxy‐dl‐aspartate, l‐cysteinesulphinate, l‐cysteate and d‐aspartate strongly inhibit the observed high affinity uptake of l‐[3H]glutamate by rat brain slices in a manner consistent with linear competitive inhibition. These substances should therefore be considered as possible substrates for the transport system. Each of these four acidic amino acids excites central neurones in a manner similar to excitation induced by l‐glutamate, and as each might occur in brain tissue, their possible synaptic role should be investigated.