Optimization of pH for the separation of organic acids in capillary zone electrophoresis.
Optimization of pH for the separation of organic acids in capillary zone electrophoresis.
复制标题
毛细管区带电泳分离有机酸的 pH 值优化。
DOI：
10.1021/ac00051a001
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发表时间：
1993
影响因子：
7.4
通讯作者：
Khaledi,MG
中科院分区：
文献类型：
--
作者：
Smith,SC;Khaledi,MG
介绍了一组取代酚的迁移行为和最佳分离。描述 CZE 中迁移率的数学模型，根据每种溶质的基本常数（解离酸 µ*- 的酸解离常数 Ku 迁移率）和缓冲液的 pH 值来预测每种溶质作为 pH 函数的迁移率。计算每个峰对之间的分辨率，并使用最小分辨率随 pH 值变化的窗口图来确定最佳 pH 值。预测和实际电泳图的比较显示了该技术的有用性。
The migration behavior and optimum separation of a set of substituted phenols Is presented. A mathematical model that describes mobility In CZE In terms of fundamental constants of each solute (acid dissociation constant Ku mobility of the dissociated acid µ*-) and the pH of the buffer was used to predict the mobility of each solute as a function of pH. The resolution between each peak pair was calculated, and a window diagram of minimum resolution as a function of pH was employed In determining the optimum pH. A comparison of the predicted and actual electropherograms shows the usefulness of this technique.