Vibrio parahaemolyticus O serotypes from O1 to O13 all produce R-type lipopolysaccharide: SDS-PAGE and compositional sugar analysis.
Vibrio parahaemolyticus O serotypes from O1 to O13 all produce R-type lipopolysaccharide: SDS-PAGE and compositional sugar analysis.
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DOI：
10.1111/j.1574-6968.1995.tb07733.x
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发表时间：
1995-08
影响因子：
2.1
通讯作者：
T. Iguchi;S. Kondo;K. Hisatsune
中科院分区：
文献类型：
--
作者：
T. Iguchi;S. Kondo;K. Hisatsune
研究了从所有O血清型副溶血性弧菌中分离的脂多糖（LPS）的分子结构。在Sephadex G-50柱上的凝胶色谱中，通过温和的酸水解从每个血清型LPS制备的降解的多糖级分仅产生核心寡糖（Frc II）和单糖（Frc III）级分，但没有对应于由聚合的重复寡糖单元组成的O多糖链的级分（Frc I）。Frc Ⅱ和Ⅲ的组成糖分析表明，所有血清型的副溶血性弧菌的LPS的糖链最多由10个单糖组成。LPS的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳没有产生类似于S型肠细菌LPS的双梯形带。这些结果与解释相符，即副溶血性弧菌O血清型从O 1到O 13都产生R型LPS，尽管形态学上光滑的外观，证明了毒力和血清学O特异性。
The molecular architecture of lipopolysaccharide (LPS) isolated from all O serotypes of Vibrio parahaemolyticus was investigated. In gel chromatography on a Sephadex G-50 column, the degraded polysaccharide fraction prepared from each serotype LPS by mild acid hydrolysis yielded only core oligosaccharide (Frc II) and monosaccharide (Frc III) fractions, but no fraction (Frc I) corresponding to O polysaccharide chain consisting of polymeric repeating oligosaccharide units. Compositional sugar analysis of Frc II and III suggested that the sugar chain of LPS of all the serotypes of V. parahaemolyticus consisted of at most ten monosaccharides. Sodium dodecylsulfate-polyacrylamide gel electrophoresis of the LPS resulted in no doublet ladder band similar to that observed for S-type enterobacterial LPS. These results are compatible with the interpretation that V. parahaemolyticus O serotypes from O1 to O13 all produce R-type LPS, despite the morphologically smooth appearance, demonstrated virulence and serological O-specificity.