Genome-wide screening and identification of nuclear Factor-Y family genes and exploration their function on regulating abiotic and biotic stress in potato (Solanum tuberosum L.)
Genome-wide screening and identification of nuclear Factor-Y family genes and exploration their function on regulating abiotic and biotic stress in potato (Solanum tuberosum L.)
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马铃薯核因子 Y 家族基因的全基因组筛选和鉴定及其调节非生物和生物胁迫的功能
DOI：
10.1016/j.gene.2021.146089
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发表时间：
2022
期刊：
影响因子：
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通讯作者：
Jun Zhao
中科院分区：
文献类型：
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作者：
Guochao Xuanyuan;Qun Lian;Ruifang Jia;Miru Du;Liru Kang;Yuanyuan Pu;Zhiwei Zhang;Jianjian Qi;Jun Zhao
核因子- y （NF-Y）转录因子（TF），包括三个不同的亚基（NF-YA， NF-YB和NF-YC），已知操纵植物生长，发育和胁迫反应的各个方面。尽管在许多物种中对theNF-Ygene家族进行了很好的研究，但对其在马铃薯中的功能知之甚少。本研究共鉴定出37个马铃薯f - ygenes，包括11个stnf - yas， 20个stnf - ybs和6个stnf - ycs。通过比较这些estnf - ygenes的进化关系、内含子/外显子组织和基序分布模式，研究了它们的遗传特征。多重比对表明，所有StNF-Y蛋白都具有明显保守的核心区域，其两侧有非保守序列。基因重复分析表明，9个stnf - y基因发生串联重复，8个stnf - y基因发生片段重复。同源性分析表明，大多数stnf - ygenes（33 / 37）与马铃薯的近亲番茄（Solanum lycopersicumL.）同源。stnf - ygenes的组织特异性表达表明它们在控制马铃薯生长发育中的潜在作用。stnf - ys在调节马铃薯对非生物胁迫（ABA、干旱和盐度）的响应中的作用也得到了证实：在不同的非生物处理下，12个nf - ys基因上调，另外2个基因下调。此外，基因对病原体挑战的反应不同，这表明stnf - ygenes可能在某些生物胁迫下发挥不同的作用。综上所述，本文对NF-Y家族成员的进化及其在马铃薯发育和胁迫反应中的作用有了深入的了解。
The Nuclear Factor-Y (NF-Y) transcription factor (TF), which includes three distinct subunits (NF-YA, NF-YB and NF-YC), is known to manipulate various aspects of plant growth, development, and stress responses. Although theNF-Ygene family was well studied in many species, little is known about their functions in potato. In this study, a total of 37 potatoNF-Ygenes were identified, including 11StNF-YAs, 20StNF-YBs, and 6StNF-YCs. The genetic features of theseStNF-Ygenes were investigated by comparing their evolutionary relationship, intron/exon organization and motif distribution pattern. Multiple alignments showed that all StNF-Y proteins possessed clearly conserved core regions that were flanked by non-conserved sequences. Gene duplication analysis indicated that nineStNF-Ygenes were subjected to tandem duplication and eightStNF-Ys arose from segmental duplication events. Synteny analysis suggested that mostStNF-Ygenes (33 of 37) were orthologous to potato’s close relative tomato (Solanum lycopersicumL.). Tissue-specific expression of theStNF-Ygenes suggested their potential roles in controlling potato growth and development. The role ofStNF-Ys in regulating potato responses to abiotic stress (ABA, drought and salinity) was also confirmed: twelveStNF-Ygenes were up-regulated and another two were down-regulated under different abiotic treatments. In addition, genes responded differently to pathogen challenges, suggesting thatStNF-Ygenes may play distinct roles under certain biotic stress. In summary, insights into the evolution of NF-Y family members and their functions in potato development and stress responses are provided.