Regeneration of canine peroneal nerve with the use of a polyglycolic acid-collagen tube filled with laminin-soaked collagen sponge: A comparative study of collagen sponge and collagen fibers as filling materials for nerve conduits
Regeneration of canine peroneal nerve with the use of a polyglycolic acid-collagen tube filled with laminin-soaked collagen sponge: A comparative study of collagen sponge and collagen fibers as filling materials for nerve conduits
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DOI：
10.1002/jbm.1061
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发表时间：
2001-12-05
期刊：
影响因子：
--
通讯作者：
Endo, K
中科院分区：
文献类型：
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作者：
Toba, T;Nakamura, T;Endo, K
研制了一种新型人工神经导管，并通过对犬周围神经80 mm间隙的再生效果进行了评价。神经导管由聚乙醇酸-胶原蛋白管制成，管内填充层粘连蛋白浸泡的胶原蛋白海绵。填充海绵状或纤维状胶原蛋白的导管被植入腓神经的80毫米间隙（每种形式5只狗）。术后12个月海绵组的神经再生在形态计量学（神经组织百分比：纤维：39.7 +/- 5.2，海绵：43.0 +/- 4.5,n = 3）和电生理（纤维：CMAP: 1.06 +/- 0.077, SEP 1.32 +/- 0.127，海绵：CMAP: 1.04 +/- 0.106, SEP 1.24 +/- 0.197, n = 5）上均优于海绵组，但差异无统计学意义。观察到的再生与先前在同一模型中报道的成功结果是互补的，其中胶原纤维完全被使用。结果表明，胶原海绵作为填充材料可能优于胶原纤维。此外，海绵的大规模可生产性，优越的支架潜力和逐渐释放可溶性因子的能力使其成为细纤维的有吸引力的替代品，细纤维在技术上困难且生产成本高。这种新开发的神经导管有潜力增强周围神经再生跨越较长的间隙通常遇到在临床设置。(C) 2001约翰威利父子公司
A novel artificial nerve conduit was developed and its efficiency was evaluated on the basis of promotion of peripheral nerve regeneration across an 80-mm gap in dogs. The nerve conduit was made of a polyglycolic acid-collagen tube filled with laminin-soaked collagen sponge. Conduits filled with either sponge- or fiber-form collagen were implanted into an 80-mm gap of the peroneal nerve (five dogs for each form). Twelve months postoperatively nerve regeneration was superior in the sponge group both morphometrically (percentage of neural tissue: fiber: 39.7 +/- 5.2, sponge: 43.0 +/- 4.5, n = 3) and electrophysiologically (fiber: CMAP 1.06 +/- 0.077, SEP 1.32 +/- 0.127 sponge: CMAP 1.04 +/- 0.106, SEP 1.24 +/- 0.197, n = 5), although these differences were not statistically significant. The observed regeneration was complementary to successful results reported previously in the same model, in which collagen fibers exclusively were used. The results indicate a possible superiority, of collagen sponge over collagen fibers as filling materials. In addition, the mass-producibility, superior scaffolding potential, and capacity for gradual release of soluble factors of the sponge provide make it an attractive alternative to fine fibers, which are both technological ly difficult and costly to produce. This newly developed nerve conduit has the potential to enhance peripheral nerve regeneration across longer gaps commonly encountered in clinical settings. (C) 2001 John Wiley & Sons, Inc.