Moist-Retaining, Self-Recoverable, Bioadhesive, and Transparent in Situ Forming Hydrogels To Accelerate Wound Healing
Moist-Retaining, Self-Recoverable, Bioadhesive, and Transparent in Situ Forming Hydrogels To Accelerate Wound Healing
复制标题
保湿、可自我恢复、生物粘附和透明原位形成水凝胶可加速伤口愈合
DOI：
10.1021/acsami.9b17180
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发表时间：
2020-01-15
影响因子：
9.5
通讯作者：
Pan, Jian-Feng
中科院分区：
文献类型：
--
作者：
Li, Jun;Yu, Fan;Pan, Jian-Feng
在加速伤口愈合的管理中，潮湿的环境起着重要作用。与其他各种形式的支架相比，水凝胶可以保持伤口区域的潮湿环境。它们是交联的亲水性聚合物网络，类似于天然软组织和细胞外基质。其中，可注射水凝胶在伤口修复中引起了极大的关注，因为它们可以注射到不规则形状的皮肤缺损中并原位形成以塑造不同尺寸的轮廓。优异的顺应性使水凝胶易于适应不同皮肤运动条件下的伤口。在这里，我们氧化羟乙基淀粉（O-HES）和改性羧甲基壳聚糖（M-CMCS），以制造一个原位形成的水凝胶具有良好的自我恢复的可拉伸性，可压缩性，生物相容性，生物降解性，和透明的加速伤口愈合。O-HES的氧化度为74%。M-CMCS的氨基化度为63%。通过Schiff碱反应制备了M-CMCS/O-HES水凝胶。结果表明，体积比为5：5的M-CMCS/O-HES水凝胶具有较好的凝胶化时间、保水性、自恢复性、生物可降解性和良好的生物相容性。然后，在具有全层皮肤缺损的Sprague道利大鼠中进行皮肤伤口愈合的实验研究。在M-CMCS/O-HES水凝胶治疗组中实现了显著结局，包括伤口闭合百分比更高、肉芽组织形成更多、上皮形成更快和胶原沉积减少。这些发现表明，使用所获得的M-CMCS/O-HES水凝胶是一种有前途的伤口愈合治疗策略。
In the management of accelerating wound healing, moist environments play an important role. Compared with other scaffolds of various forms, hydrogels can maintain a moist environment in the wound area. They are cross-linked hydrophilic polymeric networks that resemble natural soft tissues and extracellular matrices. Among them, injectable hydrogels have attracted great attention in wound repair, as they can be injected into irregular-shaped skin defects and formed in situ to shape the contour of different dimensions. The excellent compliance makes hydrogels easy to adapt to the wound under different conditions of skin movement. Here, we oxidized hydroxyethyl starch (O-HES) and modified carboxymethyl chitosan (M-CMCS) to fabricate an in situ forming hydrogel with excellent self-recoverable extensibility-compressibility, biocompatibility, biodegradability, and transparency for accelerating wound healing. The oxidation degree of O-HES was 74%. The amino modification degree of M-CMCS was 63%. M-CMCS/O-HES hydrogels were formed through the Schiff base reaction. The physicochemical properties of M-CMCS/O-HES hydrogels with various ratios were investigated, and M-CMCS/O-HES hydrogel with a volume ratio of 5:5 exhibited appropriate gelation time, notable water-retaining capacity, self-recoverable conformal deformation, suitable biodegradability, and good biocompatibility for wound-healing application. Then, skin wound-healing experimental studies were carried out in Sprague Dawley rats with full-thickness skin defects. Significant outcomes were achieved in the M-CMCS/O-HES hydrogel-treated group including higher wound closure percentage, more granulation tissue formation, faster epithelialization, and decreased collagen deposition. These findings demonstrate that using the obtained M-CMCS/O-HES hydrogels is a promising therapeutic strategy for wound healing.