Molecular characterization of two novel isoforms and a soluble form of mouse CLEC-2.
Molecular characterization of two novel isoforms and a soluble form of mouse CLEC-2.
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DOI：
10.1016/j.bbrc.2008.03.070
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发表时间：
2008-06
影响因子：
3.1
通讯作者：
Jianhui Xie;Tao Wu;Liang Guo;Yuanyuan Ruan;Lei Zhou;Haiyan Zhu;Xiao-jing Yun;Yi Hong;Jianhai Jiang;Y. Wen;J. Gu
中科院分区：
文献类型：
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作者：
Jianhui Xie;Tao Wu;Liang Guo;Yuanyuan Ruan;Lei Zhou;Haiyan Zhu;Xiao-jing Yun;Yi Hong;Jianhai Jiang;Y. Wen;J. Gu
CLEC-2首先被鉴定为具有免疫功能的C型凝集素样分子的序列相似性。最近，人CLEC-2被报道为血小板聚集性蛇毒毒素Rhodocytin和内源性唾液酸糖蛋白PodoPlanin的受体。据报道，它还有助于捕获艾滋病毒-1。然而，对小鼠CLEC-2(mCLEC-2)的研究在其鉴定后进展甚微。在本研究中，我们鉴定了mCLEC-2的两个新的剪接变异体，它们分别来自于外显子2和2/4的缺失。这两个变异体与全长mCLEC-2有不同的表达谱和亚细胞定位。此外，我们观察到全长mCLEC-2可能被对抑肽酶和PMSF敏感的蛋白酶切割成部分以二硫键连接的同源二聚体形式存在的可溶性形式。这里给出的结果代表了对mCLEC-2理解的进一步进步。
CLEC-2 was first identified by sequence similarity to C-type lectin-like molecules with immune functions. Recently, human CLEC-2 has been reported as a receptor for the platelet-aggregating snake venom toxin rhodocytin and the endogenous sialoglycoprotein podoplanin. It has also been reported to facilitate the capture of HIV-1. However, investigation of mouse CLEC-2 (mCLEC-2) has little progressed after its identification. In this study, we identified two novel splicing variants of mCLEC-2 derived from omission of exon 2 and 2/4, respectively. These two variants had different expression profiles and subcellular localization from full-length mCLEC-2. Moreover, we observed that full-length mCLEC-2 could be cleaved probably by proteases sensitive to aprotinin and PMSF into a soluble form that partially existed as a disulfide-linked homodimer. The results presented here represent a further advancement toward the understanding of mCLEC-2.