REOXYGENATION WITH 100% OXYGEN FOLLOWING HYPOXIA IN MICE CAUSES APOPTOSIS
REOXYGENATION WITH 100% OXYGEN FOLLOWING HYPOXIA IN MICE CAUSES APOPTOSIS
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DOI：
10.1097/shk.0000000000000891
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发表时间：
2017-11-01
期刊：
影响因子：
3.1
通讯作者：
Nishiyama，Takashi
中科院分区：
文献类型：
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作者：
Nishimura，Yoshiro;Ueki，Masaaki;Nishiyama，Takashi
缺氧后，空气复氧是足月新生儿的共识治疗;然而，成人高氧复氧的影响尚不清楚。本研究旨在观察缺氧后100%氧复氧对小鼠炎症和细胞凋亡的影响。8周龄小鼠在8%氧气中缺氧30分钟或空气作为对照。缺氧后，小鼠在21%或100%氧气下进行30分钟的复氧。采用组织病理学和肿瘤坏死因子-α（TNF-α）、白细胞介素-1 β（IL-1β）、半胱天冬酶-3（caspase-3）和脑源性神经营养因子（BDNF）mRNA水平检测。与21%氧相比，100%氧复氧后2.5h全脑TNF-α、IL-1β、caspase-3 mRNA水平显著升高，与21%氧复氧后9 h全脑促红细胞生成素mRNA水平显著降低。100%氧和21%氧复氧组与对照组相比，BDNF mRNA表达水平显著降低。三组中均未发现明显的神经元死亡异常结果。缺氧后100%氧复氧诱导成年小鼠炎症和细胞凋亡。因此，这些结果表明，缺氧后用100%氧复氧对成人脑以及新生儿脑都有有害影响。
After hypoxia, reoxygenation with air is the consensus treatment for full-term neonates; however, the effect of hyperoxic reoxygenation of adults is unknown. The present study was designed to investigate the effects of reoxygenation with 100% oxygen after hypoxia on inflammation and apoptosis in mice. Eight-week-old mice were either subjected to hypoxia in 8% oxygen for 30 min or air served as controls. Following hypoxia, mice underwent reoxygenation for 30 min with 21% or 100% oxygen. Tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), caspase-3 and brain derived neurotrophic factor (BDNF) mRNA study and histopathological study were performed. Reoxygenation with 100% oxygen significantly increased TNF-α (2.5 h after hypoxia), IL-1β (5 h after hypoxia), caspase-3 (8 h after hypoxia) mRNA levels in the whole brain compared with 21% oxygen, and significantly decreased erythropoietin mRNA expression compared with 21% oxygen 9 h after reoxygenation. However, reoxygenation with 100% oxygen and 21% oxygen significantly decreased BDNF mRNA levels compared with control air group. There were no clear abnormal findings showing neuronal death among the three groups. Reoxygenation with 100% oxygen after hypoxia induced inflammation and apoptosis in adult mice. Therefore, these results suggest that the reoxygenation with 100% oxygen after hypoxia has harmful effects on adult brain as well as on neonatal brain.