Fluorescence titration with apoflavodoxin: a sensitive assay for riboflavin 5'-phosphate and flavin adenine dinucleotide in mixtures.
Fluorescence titration with apoflavodoxin: a sensitive assay for riboflavin 5'-phosphate and flavin adenine dinucleotide in mixtures.
复制标题
使用脱辅基黄素氧还蛋白进行荧光滴定：混合物中核黄素 5-磷酸和黄素腺嘌呤二核苷酸的灵敏测定。
DOI：
10.1016/0003-2697(75)90656-9
复制
发表时间：
1975
影响因子：
2.9
通讯作者：
S. Mayhew
中科院分区：
文献类型：
--
作者：
J. H. Wassink;S. Mayhew
本文报道了用荧光滴定法测定混合物中核黄素5′-磷酸（FMN）和黄素腺嘌呤二核苷酸（FAD）的方法。准确的测定可以进行在存在的各种化合物，降低FMN的荧光产率;因此，该方法可能是特别有用的生物材料的粗蛋白质的无提取物的分析。
A method is described for determining riboflavin 5′-phosphate (FMN) and flavin adenine dinucleotide (FAD) in mixtures by fluorimetric titration with the FMN-specific apoprotein of flavodoxin from Peptostreptococcus elsdenii. Accurate determinations can be carried out in the presence of a variety of compounds that decrease the fluorescence yield of FMN; the method may therefore be especially useful in the analysis of crude protein-free extracts of biological materials.