Mechanical injury of explants from the articulating surface of the inner meniscus
Mechanical injury of explants from the articulating surface of the inner meniscus
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DOI：
10.1016/j.abb.2009.11.022
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发表时间：
2010-02-15
影响因子：
3.9
通讯作者：
Frisbie, David D.
中科院分区：
文献类型：
--
作者：
Kisiday, John D.;Vanderploeg, Eric J.;Frisbie, David D.
膝关节骨关节炎是加速损伤半月板，纤维软骨组织，有助于负荷传输。然而，很少有人知道半月板的机械或细胞的反应，以伤害性过载。在这里，体外研究探讨了使用压缩过载协议，已充分表征关节软骨的关节外植损伤。平行处理软骨样本，作为软骨损伤的广泛文献参考。受伤的骨外植体在关节面显示出广泛的细胞死亡，但没有明显的组织损伤，而类似的峰值应力和应变条件导致软骨表面裂缝和细胞死亡，与中度过载一致。损伤后的基因表达在骨外植体中表明，在调查的9种分解代谢和促炎分子中有7种减少，而软骨仅经历了ADAMTS-5和TNF-α的下调。这些数据表明半月板对损伤具有弹性，并且分解代谢活动的急剧增加不一定是机械过载的结果。(C)2009 Elsevier Inc. All rights reserved.
Knee osteoarthritis is accelerated by damage to the meniscus, a fibrocartilage tissue that assists in load transmission. However, little is known about the mechanical or cellular response of the meniscus to injurious overloading. Here, in vitro studies explored injury to meniscal explants using a compressive overloading protocol that has been well characterized for articular cartilage. Cartilage samples were processed in parallel as a reference to the extensive literature on cartilage injury. Injured meniscal explants showed extensive cell death at the articulating surface but no gross tissue damage, while similar conditions of peak stress and strain resulted in cartilage surface fissures and cell death consistent with moderate overloading. Post-injury gene expression in meniscal explants indicated a decrease in seven of the nine catabolic and pro-inflammatory molecules surveyed, while cartilage experienced a downregulation in ADAMTS-5 and TNF-alpha only. These data demonstrated a resiliency of the meniscus to injury, and that an acute increase in catabolic activities is not necessarily a consequence of mechanical overloading. (C) 2009 Elsevier Inc. All rights reserved.