Chemiluminescence detection of amino acids using an Edman-type reagent, 4-(1′-cyanoisoindolyl)phenylisothiocyanate
Chemiluminescence detection of amino acids using an Edman-type reagent, 4-(1′-cyanoisoindolyl)phenylisothiocyanate
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DOI：
10.1016/j.aca.2004.11.061
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发表时间：
2005-04-11
影响因子：
6.2
通讯作者：
Lu, JZ
中科院分区：
文献类型：
--
作者：
Kai, M;Morizono, M;Lu, JZ
4-(1‘-氰基异吲哚)苯基异硫氰酸酯(CIPIC)可作为化学发光(CL)Edman型试剂。首先，对化学发光反应的pH值、缓冲液类型、H_2O_2浓度、有机溶剂等条件进行了优化。其次，将丙氨酸衍生物的化学发光灵敏度与荧光法和吸光度检测法进行了比较。第三，CIPIC及其类似物的化学发光反应后的化学发光中间体和发射体的结构经LC-MS和核磁共振确证。通过氨基酸与CIPIC的偶联反应和环化反应，在信噪比为3的条件下，用化学发光法对16种氨基酸的硫代海因衍生物进行了分离和灵敏检测，检测下限为0.3~0.8pmol。(C)2004爱思唯尔B.V.保留所有权利。
The 4-(1'-cyanoisoindolyl)phenylisothiocyanate (CIPIC) could be used as a chemiluminescence (CL) Edman-type reagent. Firstly, CL reaction conditions such as pH, buffer types, H2O2 concentration and organic solvents were optimized in detail. Secondly, the CL sensitivity of the derivative of Ala was compared with fluorescence and absorbance detections. Thirdly, the structures of the CL intermediate and emitter were elucidated by liquid chromatography-mass spectrometry (LC-MS) and NMR of CIPIC and its analogues after their CL reactions. Consequently, the thiohydantoin derivatives for 16 kinds of amino acids were separated and sensitively detected by CL with lower detection limits of 0.3-0.8 pmol at a signal-to-noise ratio of 3, after the coupling reaction of amino acids with CIPIC and their cyclisation reaction. (c) 2004 Elsevier B.V. All rights reserved.