Fibreoptic fluorescent microscopy in studying biological objects
Fibreoptic fluorescent microscopy in studying biological objects
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DOI：
10.1070/qe2010v040n09abeh014344
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发表时间：
2010-01-01
影响因子：
0.9
通讯作者：
Anokhin, K. V.
中科院分区：
文献类型：
--
作者：
Morozov, A. N.;Lazutkin, A. A.;Anokhin, K. V.
The method of fluorescent microscopy is developed based on employment of a single-mode fibreoptic channel to provide high spatial resolution 3D images of large cleared biological specimens using the 488-nm excitation laser line. The transverse and axial resolution of the setup is 5 and 13 mm, respectively. The transversal sample size under investigation is up to 10 mm. The in-depth scanning range depends on the sample transparency and reaches 4 mm in the experiment. The 3D images of whole mouse organs (heart, lungs, brain) and mouse embryos obtained using autofluorescence or fluorescence of exogenous markers demonstrate a high contrast and cellular-level resolution.
