Diversity, Community Composition and Abundance of Anammox Bacteria in Sediments of the North Marginal Seas of China.
Diversity, Community Composition and Abundance of Anammox Bacteria in Sediments of the North Marginal Seas of China.
复制标题
中国北缘海沉积物中厌氧氨氧化细菌的多样性、群落组成和丰度
DOI：
10.1264/jsme2.me15140
复制
发表时间：
2016-06-25
影响因子：
2.2
通讯作者：
Zhang XH
中科院分区：
文献类型：
--
作者：
Shehzad A;Liu J;Yu M;Qismat S;Liu J;Zhang XH
在过去的几十年中，Anammox细菌被认为是在全球海洋氮循环中释放大量氮的主要参与者。通过克隆图书馆的建设和定量PCR分析，确定了中国北部海洋四个潜水区的沉积物。 16S rRNA基因和HZO基因标记表明，“ Candidatus scalindua”是所检查的16S rRNA基因clone库中的主要群体。并已知的Anammox属及其独特的系统发育位置具有较高的bootstrap值。还需要对Anammox反应进行验证。 Anammoxoglobus”，很少在研究的所有生态参数中捕获。发现细菌受到盐度，总有机物和温度的影响。沉积物样品的大小。
Over the past few decades, anammox bacteria have been recognized as key players that contribute significantly to the release of large amounts of nitrogen in the global marine nitrogen cycle. In the present study, the diversity, community composition, and abundance of anammox bacteria from the sediments of four diverse regions in the north marginal seas in China were determined via clone library construction and a quantitative PCR analysis. The clone libraries retrieved by the 16S rRNA gene and Hzo gene markers indicated that “Candidatus Scalindua” was the predominant group throughout the sites examined. The 16S rRNA gene clone libraries revealed exceptional diversity by identifying two potential novel anammox clades, as evidenced by the high sequence similarities between these two clades and known anammox genera, and their unique phylogenetic positions with high bootstrap values. However, their potential roles in the anammox reaction need to be validated. Six novel members of Planctomycetes, divergent from the known genera of anammox bacteria, were also detected. A phylogenetic analysis by Hzo protein sequences revealed the existence of two known genera, i.e., “Candidatus Jettenia” and “Candidatus Anammoxoglobus”, which are rarely captured from marine sediments. Among all ecological parameters investigated, the distribution patterns and composition of anammox bacteria were found to be influenced by salinity, total organic matter, and temperature. The abundance of the anammox bacterial 16S rRNA gene from the sites examined ranged between 3.95×105 and 9.21×105 copies g−1 wet sediment and positively correlated with the median size of the sediment sample.