Comparison of short- and long-term exposure effects of cruciferous and apiaceous vegetables on carcinogen metabolizing enzymes in Wistar rats.
Comparison of short- and long-term exposure effects of cruciferous and apiaceous vegetables on carcinogen metabolizing enzymes in Wistar rats.
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十字花科和伞形蔬菜短期和长期暴露对 Wistar 大鼠致癌物代谢酶的影响比较。
DOI：
10.1016/j.fct.2017.07.057
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Trudo,SabrinaP
中科院分区：
文献类型：
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作者：
Kim,JaeKyeom;Strapazzon,Noemia;Gallaher,CynthiaM;Stoll,DwightR;Thomas,William;Gallaher,DanielD;Trudo,SabrinaP
十字花科和豌豆科蔬菜可能是化学预防的，因为它们能够调节致癌物代谢酶，但对这些酶的影响是否会随着时间的推移而持续尚不清楚。为了检查蔬菜的短期和长期影响，大鼠被喂食四种饮食之一，持续7、30或60天：AIN-93 G、CRU（21%十字花科蔬菜-新鲜花椰菜、绿色卷心菜、豆瓣菜）、API（9%芹菜-新鲜欧洲防风、芹菜）或API + CRU（10.5% CRU + 4.5% API）。CRU在7 d后虽能增加细胞色素P450（CYP）1A 1和CYP 1A 2的活性和蛋白表达，但仅在30和60 d后仍能维持活性。有一个趋势之间的相互作用的喂养期和CRU的CYP 1A 1活性的长度;活动增加与更长的时间喂养。API增加CYP 1A 2活性，但降低磺基转移酶1A 1活性后7天，虽然不是在以后的时间。总的来说，通过CRU增加的CYP 1A活性在长期饲喂的情况下得以维持，而蛋白质量降低，这表明除了转录调控之外的机制也会产生影响。因此，反应模式和相互作用与喂养的长度可能会有所不同，这取决于蔬菜和酶的类型，在解释短期喂养研究的结果时需要谨慎。
Cruciferous and apiaceous vegetables may be chemopreventive due to their ability to modulate carcinogen-metabolizing enzymes but whether the effects on such enzymes are sustained over time is unknown. To examine the short- and long-term effects of the vegetables, rats were fed one of four diets for 7, 30, or 60 d: AIN-93G, CRU (21% cruciferous vegetables-fresh broccoli, green cabbage, watercress), API (9% apiaceous vegetables - fresh parsnips, celery), or API + CRU (10.5% CRU + 4.5% API). Although CRU increased activity and protein expression of cytochrome P450 (CYP) 1A1 and CYP1A2 after 7 d, only activity was sustained after 30 and 60 d. There was a trend towards an interaction between the length of feeding period and CRU for CYP1A1 activity; activity increased with greater time of feeding. API increased CYP1A2 activity but decreased sulfotransferase 1A1 activity after 7 d, although not at later times. Altogether, increased CYP1A activity by CRU was maintained with long term feeding while protein amount decreased, suggesting influence by mechanisms other than, or in addition to, transcriptional regulation. Thus, response patterns and interactions with length of feeding may differ, depending upon the types of vegetables and enzymes, requiring caution when interpreting the results of short-term feeding studies.