Far-red acclimating cyanobacterium as versatile source for bright fluorescent biomarkers
Far-red acclimating cyanobacterium as versatile source for bright fluorescent biomarkers
复制标题
远红驯化蓝细菌作为明亮荧光生物标志物的多功能来源
DOI：
10.1016/j.bbamcr.2018.08.015
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发表时间：
2018
影响因子：
5.1
通讯作者：
Zhao Kai-Hong
中科院分区：
文献类型：
--
作者：
Ding Wen-Long;Hou Ya-Nan;Tan Zi-Zhu;Jiang Su-Ping;Miao Dan;Losi Aba;Gaertner Wolfgang;Scheer Hugo;Zhao Kai-Hong
Far-red and near-infrared emitting chromophores extend applications of fluorescent proteins to regions of maximal transmission of most tissues, but present considerable engineering challenges. Far-red adapting cyanobacteria generate a novel set of biliproteins. One of them, ApcF2, from a thermophilic cyanobacterium was subjected to structure-guided, site-directed random and specific mutagenesis, and was screened for bright far-red emission. We report the generation of chromoproteins, termed BDFPs, that are small, bind auto-catalytically the ubiquitous biliverdin as chromophore, express well, and retain their fluorescence in mammalian cells and in the nematode,C.elegans. They are, moreover, photostable and tolerate high temperature, low pH and chemical denaturation. Homo-bichromophoric tandems of these proteins improve labeling, while hetero-bichromophoric systems with large Stokes shifts are suitable for applications like FRET, multi-channel or super-resolution microscopy. The BDFPs compare favorably to other biliproteins and provide a novel, extremely versatile labeling tool-box.
